QIAamp DSP Virus Spin Kit

For purification of viral nucleic acids from human plasma and serum

Features

  • Universal viral nucleic acid purification system
  • High-quality viral nucleic acids
  • Concentration of nucleic acids, with 20–150 µl elution volume
  • Rapid purification and minimal risk of cross contamination
QIAamp DSP Virus Spin Kit

Cat. No. / ID: 61704

For 50 preps: QIAamp MinElute Spin Columns, Buffers, Reagents, and Tubes
The QIAamp DSP Virus Spin Kit is intended for in vitro diagnostic use.

Product Details

The QIAamp DSP Virus Spin Kit provides silica-based DNA and RNA copurification. The kit is for labs that wish to process viral nucleic acids from plasma or serum for in vitro diagnostic use. The QIAamp DSP Virus Spin Kit may be used with a microcentrifuge or may be automated on the QIAcube.

Performance

The simple QIAamp DSP Virus Spin procedure, which is suitable for simultaneous processing of multiple samples, yields purified nucleic acids in less than 1 hour. Purified viral nucleic acids are ready for use in downstream applications or for storage at –20°C.

Principle

The QIAamp DSP Virus Spin Kit provides silica-based viral DNA and RNA copurification using a spin procedure.

Procedure

The QIAamp DSP Virus Spin Kit procedure includes 5 steps: lyse, bind, wash, dry spin, and elute. The kit provides rapid, reliable viral DNA and RNA purification while minimizing cross-contamination risks (see flowchart, " Procedure"). The purification can be fully automated on the QIAcube. If automating the QIAamp DSP Virus Spin Kit on the QIAcube instrument, the instrument may process fewer than 50 samples due to dead volumes, evaporation, and additional reagent consumption by automated pipetting. QIAGEN only guarantees 50 sample preps with manual use of the QIAamp DSP Virus Spin Kit.
See figures

Applications

The QIAamp DSP Virus Spin Kit provides co-purification of RNA and DNA from human plasma and serum.

Supporting data and figures

Resources

Performance Data (1)
Kit Handbooks (1)
Brochures & Guides (1)

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728