A quantitative real-time PCR was run to compare a specific single-copy human DNA target and the Investigator Quantiplex multi-copy target. The ΔCT between the two targets is shown for the different populations.
The Investigator Quantiplex HYres Kit yields results in 51 minutes on the Rotor-Gene Q and 62 minutes on the Applied Biosystems 7500 Real-Time PCR Cycler. This is significantly faster than kits from other suppliers.
Control DNA Z1 was diluted to 0.3 pg/μl and quantified using the Investigator Quantiplex Kit in 2 independent runs.