On the left, progressive substitutions of DNA nucleotides with LNA increases the melting temperature of the oligonucleotide, while maintaining the recognition sequence and specificity of the probe. On the right, LNA substitutions allow shortening of the probe, while maintaining the same Tm.
Signal intensity from microarray experiments using LNA-enhanced or DNA-based capture probes. miRNA targets with varying GC content were added at 100 amol each. The signal from DNA-based capture probes varies with GC content and results in poor detection of many miRNAs, whereas LNA probes offer robust detection of all miRNAs.