Rat liver was stored in Allprotect Reagent before total protein was purified using the Qproteome Mammalian Protein Prep Kit. Acidic phosphatase activity was similar to that of protein from control liver stored in liquid nitrogen. Each bar shows the mean from two liver pieces assayed in duplicate.
Rat lung was stored at 25°C in Allprotect Reagent or in PBS for the indicated times prior to real-time RT-PCR analysis of c-fos expression. Transcript levels relative to those of liquid nitrogen stabilized lung were calculated. The data show that Allprotect Reagent prevents c-fos induction.
Rat intestine was stored at 25°C in Allprotect Reagent or in PBS for the indicated times prior to real-time RT-PCR analysis of Madh7 expression. Transcript levels relative to those of liquid nitrogen stabilized intestine were calculated. The data show that Allprotect Reagent prevents RNA degradation.
Various rat tissues were stored in Allprotect Reagent at 25°C for 5 days prior to western blot analysis of ERK2. Blot intensities were similar to those from liquid nitrogen stabilized tissues, indicating no protein degradation.
Human intestinal mucosa samples were stored for 1 day in Allprotect Reagent (4°C) or in liquid nitrogen, and then analyzed by mass spectrometry. The Allprotect samples gave similar peak patterns for [A] <10 kDa and [B] 10–20 kDa protein as the liquid nitrogen samples, indicating stabilization of the protein content. Example analyses are shown. (Data kindly provided by Indivumed GmbH, Hamburg, Germany).
Each Allprotector molecule possesses contact points that allow multiple Allprotector molecules to envelop and protect DNA, RNA, and protein. Upon tissue lysis, Allprotector molecules become diluted and fall apart, releasing DNA, RNA, and protein.
