QIAseq FastSelect Kits

For rapid removal of ribosomal and other RNAs during RNA-seq library construction for NGS

Features

  • High-performance rRNA removal in just 14 minutes
  • Start with fragmented or high-quality RNA
  • Novel RNA removal mechanism doesn't involve hybrid capture or enzymatic digestion
  • Combine FastSelect kits together for mixed samples
  • Compatible with QIAGEN, Illumina, NEB, KAPA/Roche and other RNA-seq library kits
QIAseq FastSelect –Globin Kit (24)

Cat. No. / ID: 334376

Includes 3 tubes of QIAseq FastSelect reagent for globin mRNA removal; sufficient for 24 reactions from human, mouse and rat samples
For
Globin mRNA removal
rRNA removal
rRNA/Globin mRNA removal
Samples
24
96

Product Details

QIAseq FastSelect Kits remove unwanted RNAs from RNA-seq libraries using a single, one-step, 14-minute protocol which integrates seamlessly into RNA-seq library workflows. QIAseq FastSelect was designed to work with highly fragmented RNA with low RIN values or high-quality RNA and is compatible with RNA-seq library kits from QIAGEN, NEB, KAPA/Roche and others.

We recommend using QIAseq Stranded RNA Library Kits for robust strand-specific RNA-seq library preparation from high-quality and highly fragmented (FFPE) RNA. QIAseq Stranded RNA Library Kits utilize unique dual indexing (UDIs) which enables multiplexing of up to 384 samples per flow cell lane on Illumina NGS instruments and mitigates index hopping on Illumina NovaSeq and Illumina patterned flow cells.

If you are interested in removing more RNAs, consider building a QIAseq FastSelect Custom Kit in GeneGlobe. Contact us!

Want to try the QIAseq FastSelect –rRNA/Globin Kit for the first time? Request a trial kit to evaluate.

Want to try the QIAseq FastSelect –5S/16S/23S Kit for the first time? Request a trial kit to evaluate.

Want to try the QIAseq FastSelect –rRNA Yeast Kit for the first time? Request a trial kit to evaluate.

Want to try the QIAseq FastSelect –rRNA Plant Kit for the first time? Request a trial kit to evaluate

Performance

QIAseq FastSelect –rRNA HMR Kits demonstrated highly efficient removal of rRNA and superior performance compared to our previous kit versions and alternative products.

See figures:

 QIAseq FastSelect –rRNA HMR Kit removes rRNA with 1 step in 14 minutes

 QIAseq FastSelect –rRNA HMR Kit has comprehensive coverage to remove cytoplasmic and mitochondrial rRNA targets

 QIAseq FastSelect –Globin Kit has comprehensive coverage to remove adult, fetal and embryonic globin mRNA RNA

 QIAseq FastSelect –rRNA HMR Kit provides greater rRNA removal than previous kit versions

 QIAseq FastSelect –rRNA HMR Kit shows comparable gene expression results with previous kit versions

 QIAseq FastSelect –rRNA HMR Kit maintains the expression profile: detection of expressed genes (RPKM >0.3)

 QIAseq FastSelect–rRNA HMR Kit maintains the expression profile: consistent detection of expressed genes (RPKM >0.3)

Robust performance with a variety of sample types, regardless of RNA input

QIAseq FastSelect provides reliable rRNA removal, consistent read mapping and reproducible gene expression results with RNA extracted from various sample types, including FFPE, and over a broad range of RNA input amounts.

See figures:

 QIAseq FastSelect –rRNA HMR Kit is a single solution that removes mitochondrial and cytoplasmic rRNA from human, mouse, rat and other mammalian samples

 QIAseq FastSelect –rRNA HMR Kit provides consistent distribution of read mapping between replicates

 QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly efficient and reproducible removal of rRNA and globin mRNA, regardless of RNA input

 QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly consistent breakdown of mapped reads, regardless of RNA input

 QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: gene expression results

 QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples

 QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: removal of rRNA leads to increased exon mapping

 QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: gene expression results

 QIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samples

 QIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samples: gene expression results

QIAseq FastSelect –5S/16S/23S Kits

In accordance with QIAGEN’s ISO-certified Quality Management System, each lot of the QIAseq FastSelect –5S/16S/23S Kit is tested against predetermined specifications to ensure consistent product quality.

QIAseq FastSelect –5S/16S/23S Kits provide robust rRNA removal from a variety of sample types.

See figures:

 QIAseq FastSelect –5S/16S/23S: robust rRNA removal from gut community sample

 QIAseq FastSelect –5S/16S/23S: robust rRNA removal from bacterial community (28 strains)

 QIAseq FastSelect –5S/16S/23S: robust rRNA removal from bacterial community: mapping analysis (28 strains)

QIAseq FastSelect –5S/16S/23S Kits deliver highly efficient and reproducible rRNA removal.

See figures:

 QIAseq FastSelect –5S/16S/23S: robust, reproducible rRNA removal from gut microbiome

 QIAseq FastSelect –5S/16S/23S: robust, reproducible rRNA removal from gut microbiome: gene expression results

 Reproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S

 Reproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S: gene expression results

 Reproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S: gene expression results (100 ng, 1 µg)

QIAseq FastSelect –5S/16S/23S Kits demonstrated highly efficient removal of rRNA and superior performance compared to alternative methods.

See figures:

 QIAseq FastSelect –5S/16S/23S vs. other methods: rRNA removal

 QIAseq FastSelect –5S/16S/23S vs. other methods: sequencing reads

 QIAseq FastSelect –5S/16S/23S vs. other methods: total reads mapped to rRNA

QIAseq FastSelect –5S/16S/23S Kits are compatible with a wide range of RNA-seq library kits.

See figures:

 QIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB

 QIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB: gene detection

 QIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB: gene expression

QIAseq FastSelect –rRNA Yeast Kits

QIAseq FastSelect –rRNA Yeast Kits demonstrated highly efficient removal of rRNA (see figure “ QIAseq FastSelect –rRNA Yeast Kits provide highly efficient removal of rRNA”) and robust performance (see figure “ QIAseq FastSelect ‒rRNA Yeast dramatically improves the number of genes detected”).

QIAseq FastSelect –rRNA Yeast Kits also provide consistent read mapping (see figures “ QIAseq FastSelect –rRNA Yeast exon/intron/intergenic mapping”, “ QIAseq FastSelect ‒rRNA Yeast dramatically shifts read allocation from rRNA to genes” and “ QIAseq FastSelect ‒rRNA Yeast dramatically shifts read allocation from rRNA to genes: bar chart”) and reproducible gene expression results (see figure “ QIAseq FastSelect ‒rRNA Yeast reproducibility”).

QIAseq FastSelect –rRNA Plant Kits

QIAseq FastSelect –rRNA Plant Kits demonstrated highly efficient removal of rRNA (see figure  “QIAseq FastSelect –rRNA Plant Kits are broadly compatible with different plants”) and robust performance (see figure “ QIAseq FastSelect ‒rRNA Plant dramatically improves the number of genes detected”).

QIAseq FastSelect –rRNA Plant Kits also provide reproducible gene expression results (see figure “ QIAseq FastSelect ‒rRNA Plant reproducibility”) and consistent read mapping with a variety of plants.

See figures:

 QIAseq FastSelect –rRNA Plant exon/intron/intergenic mapping

 QIAseq FastSelect ‒rRNA Plant dramatically shifts read allocation from rRNA to genes: Arabidopsis

 QIAseq FastSelect ‒rRNA Plant dramatically shifts read allocation from rRNA to genes: corn

 QIAseq FastSelect ‒rRNA Plant dramatically shifts read allocation from rRNA to genes: rice

 QIAseq FastSelect ‒rRNA Plant dramatically shifts read allocation from rRNA to genes: soybean

 QIAseq FastSelect ‒rRNA Plant dramatically shifts read allocation from rRNA to genes: cotton

 QIAseq FastSelect ‒rRNA Plant dramatically shifts read allocation from rRNA to genes: bar chart

See figures
QIAseq FastSelect –rRNA HMR Kit removes rRNA with 1 step in 14 minutesQIAseq FastSelect –rRNA HMR Kit removes rRNA with 1 step in 14 minutesQIAseq FastSelect –rRNA HMR Kit has comprehensive coverage to remove cytoplasmic and mitochondrial rRNA targetsQIAseq FastSelect –rRNA HMR Kit has comprehensive coverage to remove cytoplasmic and mitochondrial rRNA targetsQIAseq FastSelect –Globin Kit has comprehensive coverage to remove adult, fetal and embryonic globin mRNAQIAseq FastSelect –Globin Kit has comprehensive coverage to remove adult, fetal and embryonic globin mRNAQIAseq FastSelect –rRNA HMR Kit provides greater rRNA removal than previous kit versionsQIAseq FastSelect –rRNA HMR Kit provides greater rRNA removal than previous kit versionsQIAseq FastSelect –rRNA HMR Kit shows comparable gene expression results with previous kit versionsQIAseq FastSelect –rRNA HMR Kit shows comparable gene expression results with previous kit versionsQIAseq FastSelect –rRNA HMR Kit maintains the expression profile: detection of expressed genes (RPKM >0.3)QIAseq FastSelect –rRNA HMR Kit maintains the expression profile: detection of expressed genes (RPKM >0.3)QIAseq FastSelect–rRNA HMR Kit maintains the expression profile: consistent detection of expressed genes (RPKM >0.3)QIAseq FastSelect–rRNA HMR Kit maintains the expression profile: consistent detection of expressed genes (RPKM >0.3)QIAseq FastSelect –rRNA HMR Kit is a single solution that removes mitochondrial and cytoplasmic rRNA from human, mouse, rat and other mammalian samplesQIAseq FastSelect –rRNA HMR Kit is a single solution that removes mitochondrial and cytoplasmic rRNA from human, mouse, rat and other mammalian samplesQIAseq FastSelect –rRNA HMR Kit provides consistent distribution of read mapping between replicatesQIAseq FastSelect –rRNA HMR Kit provides consistent distribution of read mapping between replicatesQIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly efficient and reproducible removal of rRNA and globin mRNA, regardless of RNA inputQIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly efficient and reproducible removal of rRNA and globin mRNA, regardless of RNA inputQIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly consistent breakdown of mapped reads, regardless of RNA inputQIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly consistent breakdown of mapped reads, regardless of RNA inputQIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: gene expression resultsQIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: gene expression resultsQIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samplesQIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samplesQIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: removal of rRNA leads to increased exon mappingQIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: removal of rRNA leads to increased exon mappingQIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: gene expression resultsQIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: gene expression resultsQIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samplesQIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samplesQIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samples: gene expression resultsQIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samples: gene expression resultsQIAseq FastSelect –5S/16S/23S: robust rRNA removal from gut community sampleQIAseq FastSelect –5S/16S/23S: robust rRNA removal from gut community sampleQIAseq FastSelect –5S/16S/23S: robust rRNA removal from bacterial community (28 strains)QIAseq FastSelect –5S/16S/23S: robust rRNA removal from bacterial community (28 strains)QIAseq FastSelect –5S/16S/23S: robust rRNA removal from bacterial community: mapping analysis (28 strains)QIAseq FastSelect –5S/16S/23S: robust rRNA removal from bacterial community: mapping analysis (28 strains)QIAseq FastSelect –5S/16S/23S: robust, reproducible rRNA removal from gut microbiomeQIAseq FastSelect –5S/16S/23S: robust, reproducible rRNA removal from gut microbiomeQIAseq FastSelect –5S/16S/23S: robust, reproducible rRNA removal from gut microbiome: gene expression resultsQIAseq FastSelect –5S/16S/23S: robust, reproducible rRNA removal from gut microbiome: gene expression resultsReproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23SReproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23SReproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S: gene expression resultsReproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S: gene expression resultsReproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S: gene expression results (100 ng, 1 µg)Reproducible rRNA removal from E. coli K12 using QIAseq FastSelect –5S/16S/23S: gene expression results (100 ng, 1 µg)QIAseq FastSelect –5S/16S/23S vs. other methods: rRNA removalQIAseq FastSelect –5S/16S/23S vs. other methods: rRNA removalQIAseq FastSelect –5S/16S/23S vs. other methods: sequencing readsQIAseq FastSelect –5S/16S/23S vs. other methods: sequencing readsQIAseq FastSelect –5S/16S/23S vs. other methods: total reads mapped to rRNAQIAseq FastSelect –5S/16S/23S vs. other methods: total reads mapped to rRNAQIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEBQIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEBQIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB: gene detectionQIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB: gene detectionQIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB: gene expressionQIAseq FastSelect –5S/16S/23S is compatible with a wide range of RNA-seq library kits, including QIAGEN, Illumina and NEB: gene expressionT_0078_QIAseqFastSelectYeastT_0078_QIAseqFastSelectYeastBC_1038_QIAseqFastSelectYeast_Original_51522BC_1038_QIAseqFastSelectYeast_Original_51522BC_1036_QIAseqFastSelectYeast_Original_51520BC_1036_QIAseqFastSelectYeast_Original_51520PiC_0052_QIAseqFastSelectYeastPiC_0052_QIAseqFastSelectYeastBC_1037_QIAseqFastSelectYeast_Original_51521BC_1037_QIAseqFastSelectYeast_Original_51521SP_0127_QIAseqFastSelectYeast_Original_51525SP_0127_QIAseqFastSelectYeast_Original_51525T_0077_QIAseqFasrSelectPlant_Original_51518T_0077_QIAseqFasrSelectPlant_Original_51518BC_1035_QIAseqFasrSelectPlant_Original_51512BC_1035_QIAseqFasrSelectPlant_Original_51512SP_0126_QIAseqFastSelectPlant_Original_51519SP_0126_QIAseqFastSelectPlant_Original_51519BC_1033_QIAseqFasrSelectPlant_Original_51510BC_1033_QIAseqFasrSelectPlant_Original_51510PiC_0047__QIAseqFasrSelectPlant_Original_51513PiC_0047__QIAseqFasrSelectPlant_Original_51513PiC_0048_QIAseqFasrSelectPlant_Original_51514PiC_0048_QIAseqFasrSelectPlant_Original_51514PiC_0049_QIAseqFasrSelectPlant_Original_51515PiC_0049_QIAseqFasrSelectPlant_Original_51515PiC_0050_QIAseqFasrSelectPlant_Original_51516PiC_0050_QIAseqFasrSelectPlant_Original_51516PiC_0051_QIAseqFasrSelectPlant_Original_51517PiC_0051_QIAseqFasrSelectPlant_Original_51517BC_1034_QIAseqFasrSelectPlant_Original_51511BC_1034_QIAseqFasrSelectPlant_Original_51511

Principle

QIAseq FastSelect Kits remove highly expressed, but biologically unimportant, RNA transcripts during RNA-seq library construction to enable higher sensitivity and sample throughput. QIAseq FastSelect reagents limit cDNA synthesis of unwanted RNAs during the reverse transcription process regardless of the RNA quality and RNA integrity number (RIN). When incorporating FastSelect in an existing workflow, researchers only need to add FastSelect prior to the reverse transcription reaction. If you are working with mixed samples, where there may be bacteria, yeast and host (i.e., human) RNA present, simply combine the FastSelect Kits together to increase the range of RNA removal.

QIAseq FastSelect can accommodate RNA library starting amounts from 20 ng to 1 µg, and is compatible with low-quality, highly fragmented or high-quality full-length RNA. Each QIAseq FastSelect Kit has been experimentally verified for performance against its targets using RNA-seq library kits from QIAGEN, NEB, KAPA/Roche and Illumina and other workflows. Since QIAseq FastSelect does not use enzymatic digestion or hybrid-capture procedures, the fast and simple workflow results in reliable rRNA removal and high reproducibility in downstream applications. QIAseq FastSelect procedures can be set up at room temperature and easily automated, saving time and increasing lab productivity.

QIAseq FastSelect –rRNA HMR Kits remove cytoplasmic and mitochondrial rRNA, while researchers starting with blood samples can use QIAseq FastSelect –rRNA/Globin to remove both rRNA and globin mRNA. For researchers who are enriching for mRNA from blood, and only want to remove globin mRNA, we recommend QIAseq FastSelect –Globin.

QIAseq FastSelect –5S/16S/23S is a pan-bacterial ribosomal RNA (rRNA) removal kit designed to remove 5S, 16S and 23S rRNA from complex bacterial community samples. Our comprehensive rRNA removal reagent was designed using 16S (nearly 600,000 entries), 23S (nearly 170,000 entries) and 5S rRNA sequences (over 7200 entries). In silico modeling predicts >95% rRNA removal of all known 5S, 16S and 23S rRNA sequences.

QIAseq FastSelect –rRNA Yeast Kits remove cytoplasmic (35S [made up of 25S, 18S and 5.8S], 25S, 18S, 5.8S and 5S) and mitochondrial (21S, 15S, ACI60_gr01 [large subunit ribosomal RNA] and ACI60_gr02 [small subunit ribosomal RNA]) rRNA from Saccharomyces cerevisiae and Schizosaccharomyces pombe and related yeast strains.

QIAseq FastSelect −rRNA Plant removes cytoplasmic (5.8S, 18S and 25S), mitochondrial (5S, 18S and 26S) and chloroplast (4.5S, 5S, 16S and 23S) rRNA from Arabidopsis thaliana and Arabidopsis lyrata. In addition to Arabidopsis total RNA, QIAseq FastSelect −rRNA Plant has been tested with a variety of other types of plants such as barley, corn, cotton, flaxseed, maple, oat, potato, rice, rye, sorghum, soybean and wheat (see figure “QIAseq FastSelect –rRNA Plant Kits are broadly compatible with different plants”).

Procedure

Most RNA removal or depletion strategies associated with RNA-seq library construction are sample pre-treatment strategies involving hybrid-capture or enzymatic removal of unwanted RNA. Our unique QIAseq FastSelect procedure is compatible with QIAGEN, Illumina, KAPA and NEB stranded library preparation kits and provides complete rRNA removal in a single, 14-minute inline step (see figure “QIAseq FastSelect Kit workflow”). This is dramatically faster than alternative RNA depletion kits, which require pre-treatment protocols involving more than 25 steps and 2 hours to complete.

Simply add QIAseq FastSelect reagent to the RNA sample, perform fragmentation (if required), cool the reaction stepwise from 75°C to 25°C for 14 minutes and then complete the remaining library preparation steps. QIAseq FastSelect works with or without RNA fragmentation, providing the flexibility to use RNA from degraded RNA samples, or high-quality RNA as part of a standard RNA-seq library construction workflow.

QIAseq FastSelect –5S/16S/23S seamlessly integrates with your existing RNA-seq library preparation, providing RNA removal in a single, 14-minute step, which is followed by bead cleanup (figure QIAseq FastSelect –5S/16S/23S workflow). Prior to RNA heat fragmentation (which is optional and dependent upon the library preparation kit and sample type), QIAseq FastSelect –5S/16S/23S removal reagent is directly combined with total RNA and fragmentation buffer. After optional fragmentation, the reaction temperature is stepwise cooled from 75°C to 25°C over 14 minutes, followed by a bead cleanup. The RNA is then ready for reverse transcription.

See figures

Applications

QIAseq FastSelect Kits are available in a variety of different formats and sizes to suit your specific applications.

  • QIAseq FastSelect −rRNA HMR and −Globin delivers rapid, reliable RNA removal from FFPE and fresh sample RNA sources.
  • QIAseq FastSelect –5S/16S/23S delivers rapid, reliable rRNA removal for bacterial isolates, as well as complex bacterial communities.
  • QIAseq FastSelect delivers rapid, reliable RNA removal from degraded RNA and fresh sample RNA sources.
  • QIAseq FastSelect delivers rapid, reliable RNA removal from full length or fragmented RNA samples from many species of plants.

Supporting data and figures