QIAseq FastSelect Kits remove highly expressed, but biologically unimportant, RNA transcripts during RNA-seq library construction to enable higher sensitivity and sample throughput. QIAseq FastSelect reagents limit cDNA synthesis of unwanted RNAs during the reverse transcription process regardless of the RNA quality and RNA integrity number (RIN). When incorporating FastSelect in an existing workflow, researchers only need to add FastSelect prior to the reverse transcription reaction. If you are working with mixed samples, where there may be bacteria, yeast and host (i.e., human) RNA present, simply combine the FastSelect Kits together to increase the range of RNA removal.
QIAseq FastSelect can accommodate RNA library starting amounts from 20 ng to 1 µg, and is compatible with low-quality, highly fragmented or high-quality full-length RNA. Each QIAseq FastSelect Kit has been experimentally verified for performance against its targets using RNA-seq library kits from QIAGEN, NEB, KAPA/Roche and Illumina and other workflows. Since QIAseq FastSelect does not use enzymatic digestion or hybrid-capture procedures, the fast and simple workflow results in reliable rRNA removal and high reproducibility in downstream applications. QIAseq FastSelect procedures can be set up at room temperature and easily automated, saving time and increasing lab productivity.
QIAseq FastSelect –rRNA HMR Kits remove cytoplasmic and mitochondrial rRNA, while researchers starting with blood samples can use QIAseq FastSelect –rRNA/Globin to remove both rRNA and globin mRNA. For researchers who are enriching for mRNA from blood, and only want to remove globin mRNA, we recommend QIAseq FastSelect –Globin.
QIAseq FastSelect –5S/16S/23S is a pan-bacterial ribosomal RNA (rRNA) removal kit designed to remove 5S, 16S and 23S rRNA from complex bacterial community samples. Our comprehensive rRNA removal reagent was designed using 16S (nearly 600,000 entries), 23S (nearly 170,000 entries) and 5S rRNA sequences (over 7200 entries). In silico modeling predicts >95% rRNA removal of all known 5S, 16S and 23S rRNA sequences.
QIAseq FastSelect –rRNA Yeast Kits remove cytoplasmic (35S [made up of 25S, 18S and 5.8S], 25S, 18S, 5.8S and 5S) and mitochondrial (21S, 15S, ACI60_gr01 [large subunit ribosomal RNA] and ACI60_gr02 [small subunit ribosomal RNA]) rRNA from Saccharomyces cerevisiae and Schizosaccharomyces pombe and related yeast strains.
QIAseq FastSelect −rRNA Plant removes cytoplasmic (5.8S, 18S and 25S), mitochondrial (5S, 18S and 26S) and chloroplast (4.5S, 5S, 16S and 23S) rRNA from Arabidopsis thaliana and Arabidopsis lyrata. In addition to Arabidopsis total RNA, QIAseq FastSelect −rRNA Plant has been tested with a variety of other types of plants such as barley, corn, cotton, flaxseed, maple, oat, potato, rice, rye, sorghum, soybean and wheat (see figure “QIAseq FastSelect –rRNA Plant Kits are broadly compatible with different plants”).