View Slides
Learn more about QIAseq FastSelect –rRNA HMR Kits
Learn more about QIAseq FastSelect –5S/16S/23S Kits
Want to try this solution for the first time?
Request a trial kit to evaluate
Metagenomic and transcriptomic analysis from complex bacterial samples is gaining popularity due to the ease of obtaining samples and the decreased costs of next generation sequencing. While DNA profiling using 16S profile or even shot-gun genomic sequencing has become routine, RNA profiling presents a special challenge due to prokaryotic 5S/16S/23S rRNA contamination and its removal that is essential for obtaining relevant gene expression data.
For complex bacterial samples, continued evolution of our first generation QIAseq FastSelect technology recently resulted in a new strategy that is extremely efficient in removing 5S/16S/23S rRNA. We designed QIAseq FastSelect -rRNA Removal 5S-16S-23S against SILVA 16S sequences (nearly 600,000 entries), SILVA 23S sequences (nearly 170,000 entries) and 5S rRNA Database (over 7,200 entries) and theoretically block greater than 95% of all 5S, 16S and 23 rRNA databased sequences. Use of the QIAseq FastSelect -rRNA Removal 5S-16S-23S Kit with our latest mammalian QIAseq FastSelect -rRNA Removal HMR kit allows researchers to address complex samples from humans, mouse, rats and other mammalian samples without changing their workflow.
Join QIAGEN’s RNA-seq R&D team for a discussion on how we developed and benchmarked QIAseq QIAseq FastSelect 5S-16S-23S using both high quality and severely fragmented RNA samples from complex samples sources, followed by Q&A with the experts.