Extracting High-Quality Nucleic Acids from Challenging Tissue Samples

In this webinar we will present protocols and data for automated RNA and DNA extraction from tissue fixed with standard formalin or with the innovative formalin-free PAXgene Tissue System. We will demonstrate nucleic acid extraction from fixed tissue, from fibrous, fatty and enzyme-rich tissues, and from sections of paraffin-embedded tissue (FFPE and PFPE).

FFPE tissues are still the standard for histomorphological analysis. However, cross-linking is problematic for downstream molecular analyses, and formaldehyde carcinogenicity is serious environmental risk. Cryopreserved tissue specimens offer the possibility for sophisticated molecular analysis, but the histomorphology is less well-preserved, and handling is more complicated. We will present alternative solutions that address these challenges. We will also discuss the benefits of automating nucleic acid purification on the QIAcube and provide options for analyzing histomorphology from the same sample.

Dr. Daniel Groelz, Scientific Associate Director R&D, QIAGEN GmbH

Dr. Daniel Groelz is a Scientific Associate Director of Research and Development at QIAGEN in Hilden, Germany. Dr. Groelz is the R&D project manager who led an international team within PreAnalytiX GmbH to develop the PAXgene Tissue System. He was the leader for the evaluation of the PAXgene Tissue System within the European collaborative grant project, SPIDIA, and is currently managing work packages in two other grant projects to evaluate the PAXgene Tissue System for NGS applications and use in routine pathology workflows.

Prior to joining QIAGEN, Dr. Groelz worked for Autoimmun Diagnostika GmbH in Albstadt, Germany. Dr. Groelz received a Ph.D. in Biology from the University of Mainz with a concentration in Physiological Chemistry. He is the main inventor of the PAXgene Tissue System and he is an inventor/co-inventor on three other patent families.