V-J usage heatmaps for a single sample are shown. Jurkat RNA was spiked into normal human PBMC RNA at a dilution of 1:10,000 (800 Jurkat cells in 8,000,000 PBMCs).
The heatmaps allow for easy identification of enriched clonotypes across the sample. Here, we see the major clonotype of the Jurkat cell, as well as the diversity of the PBMC background.
The TCR richness of each receptor per sample is revealed.