Ninety-six leaf samples were collected from a batch of wheat plants grown under identical conditions. Samples (50 mg) were disrupted in liquid nitrogen and DNA was isolated using the DNeasy 96 Plant procedure.
Genomic DNA purified from different plant species was amplified by PCR. Eluate (5 µl) was used as template, and 30 µl of each PCR product were loaded per lane. M: markers.
Genomic DNA was purified from different plant species following disruption in liquid nitrogen. DNA was eluted in 2 x 100 µl Buffer AE. Eluates (10 µl) were subjected to gel electrophoresis. Average yields are given under the plant names. M: lambda–HindIII.