Easy high-resolution analysis
Genotyping organisms, PCR-based cDNA library screening, and many other biomedical research and molecular biology applications require discrimination of PCR fragments that vary in size by a small number of base pairs. The QIAxcel High Resolution Kit, in combination with QIAxcel instruments, allows analysis of DNA fragments between 15 bp and 5 kb in size. Fragments smaller than 500 bp can be separated with a resolution of 3–5 bp, providing greater confidence in data interpretation than is possible with conventional high-resolution agarose gel electrophoresis (see figure Sensitive detection of a mutated cancer-related gene
). At a detection sensitivity of 0.1 ng/µl DNA in undiluted amplification reactions, QIAxcel instruments offer more robust results with less sample input material.
The QIAxcel DNA Screening Kit is highly suited for routine evaluation of fragments for restriction endonuclease digestion, qualitative singleplex or multiplex PCR, and other DNA applications (see figure High-resolution analysis of multiplex PCR samples
). The use of gel cartridges for DNA screening streamlines the lab workflow by minimizing analysis time for fragments of 15 bp to 5 kb in size - 96 samples are processed in approximately 1 hour. A resolution of 20–50 bp can be obtained for fragments smaller than 1 kb. Sample consumption is less than 0.1 µl, leaving virtually all of your precious sample for further downstream use.
The QIAxcel DNA Fast Analysis Kit is highly suited for routine evaluation of DNA fragments in qualitative single or multiplex PCR applications. Use of gel cartridges for DNA screening streamlines the lab workflow by minimizing analysis time for fragments of 15 bp to 3 kb in size — 12 samples are processed in just 3 minutes and 96 samples in approximately 25 minutes (see figure Screenshot showing data from PCR fragment analysis
). A resolution of down to 50 bp can be achieved with the QIAxcel DNA Fast Analysis gel cartridge, while sample consumption is less than 0.1 μl.