The sequence indicated is the analyzed sequence for a normal sample. FP and FPB: Forward PCR primers (B indicates biotinylation); RP and RPB: Reverse PCR primers (B indicates biotinylation); Seq: Sequencing primers.
The mutation GGT to TGT was reanalyzed with the "Sequence to Analyze" NGTGRCGTAGGC targeting base 1 in codon 12 (nucleotide 34).
Nucleotide 35 indicated with an arrow.
Linearity of mutation GGT->TGT in codon 12.
The Pyrogram shows the mutation (nucleotide 34 indicated with an arrow) with the "Sequence to Analyze" GNTGRCGTAGGC targeting base 2 in codon 12 (nucleotide 35). A yellow color indicates that this sequence is unexpected and needs to be checked.