text.skipToContent text.skipToNavigation

RNeasy Protect Mini Kit

For stabilization and purification of up to 100 µg total RNA from tissues
  • Immediate RNA stabilization and protection
  • Reliable gene expression and gene-profiling data
  • No need for liquid nitrogen, dry ice, or phenol
  • Ready-to-use, high-quality total RNA in minutes
  • No CsCl gradients, no LiCl or ethanol precipitation

The RNeasy Protect Mini Kit includes RNAprotect Tissue Reagent for stabilizing RNA in tissue samples, and RNeasy spin columns for purifying up to 100 µg of high-quality RNA using silica-membrane technology. RNA purification can be automated on the QIAcube. Efficient disruption of tissue samples can be achieved using a TissueRuptor or TissueLyser system.

Buy Products

Cat No./ID: 74124
RNeasy Protect Mini Kit (50)
€410.00
Order Product

RNAprotect Tissue Reagent (50 ml), 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers

Cat No./ID: 74126
RNeasy Protect Mini Kit (250)
€1,774.00
Order Product

RNAprotect Tissue Reagent (250 ml), 250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers

The RNeasy Protect Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

0
Prevention of degradation of mRNA in tissues.
RNA was isolated from fresh rat kidney samples after 0, 5, 10, 15, 30, and 60 minutes, using either standard procedures (Unstabilized) or RNeasy Protect Kits (Stabilized). The RNA isolated was analyzed by agarose gel electrophoresis and expression of GAPDH was examined using northern blot analysis. M: markers.
1
Stable RNA in tissues.
2
Changes in mRNA levels.
Once a biological sample is harvested, its RNA becomes extremely unstable. Gene induction or down-regulation triggered by sample manipulation must be prevented to enable accurate gene expression analysis.
3
RNeasy Mini spin column.
For purification of total RNA from formalin-fixed, paraffin-embedded tissue sections.
Performance

The RNA-stabilizing properties of RNAprotect Tissue Reagent prevent gene induction or down-regulation triggered by sample manipulation, and allow purification of high-quality RNA (see figure "Prevention of degradation of mRNA in tissues"). In addition, RNeasy purified RNA from RNAprotect stabilized samples remains intact following storage of tissue samples under a wide variety of conditions (see figure "Stable RNA in tissues"). A range of tissue types can be processed with the RNeasy Protect Mini Kit (see table).

Total RNA yields obtained with RNeasy Protect Mini Kit
Mouse tissue Amount (mg) Average yield* (µg)
Brain 10 10
Liver 10 43
Lung 10 12
Spleen 10 45
* Amounts of RNA can vary due to developmental stage, species, and growth conditions of samples.

RNA purified with RNeasy technology has A260/A280 ratios of 1.9–2.1 (measured in 10 mM Tris·Cl, pH 7.5). Since the RNeasy procedure enriches for RNA species >200 nt, RNA yield does not include 5S rRNA, tRNAs, or other low-molecular-weight RNAs.

Principle

The RNeasy Protect system provides a complete RNA protection and isolation solution, from sample harvesting to pure RNA, in one kit. Proven RNeasy silica-membrane technology in spin-column format, combined with the RNA-stabilizing properties of RNAprotect Tissue Reagent, allows purification of high-quality, intact RNA. Once a biological sample is harvested, its RNA becomes extremely unstable (see figure "Changes in mRNA levels"). The innovative, aqueous RNAprotect Tissue Reagent quickly permeates tissues, stabilizing and protecting the RNA expression pattern. Samples can be archived without risk of RNA degradation, even after multiple freeze–thaw cycles. Following stabilization, RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification (see figure "RNeasy Mini spin column").

Procedure

Samples can be stabilized and stored in RNAprotect Tissue Reagent, included in RNeasy Protect Kits. For RNA purification, samples (0.5–30 mg tissue) are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the RNeasy silica membrane (binding capacity up to 100 µg RNA). RNA binds, and all contaminants are efficiently washed away. Pure, concentrated RNA is eluted in 30–100 µl water (see flowchart "RNeasy Protect Mini procedure"). RNA purification can be automated on the QIAcube.

Applications

RNA purified with RNeasy technology is ideal for use in all applications. Downstream applications include:

  • Northern, dot, and slot blotting
  • End-point RT-PCR
  • Quantitative, real-time RT-PCR
  • Array analysis
  • Poly A+ RNA selection

Specifications

Features
Specifications
Applications PCR, qPCR, real-time RT-PCR, microarray
Elution volume 30–100 µl
Format Spin column
Main sample type Tissue samples
Processing Manual (centrifugation)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein RNA
Sample amount 0.5–30 mg
Stabilization Yes
Technology Silica technology
Yield 10 –45 µg

Product Resources

You are not authorized to download the resource

FAQs (35)
FAQ ID -12
View
FAQ ID -32
View
FAQ ID -97
View
FAQ ID -101
View
FAQ ID -103
View
FAQ ID -113
View
FAQ ID -139
View
FAQ ID -159
View
FAQ ID -1023
View
FAQ ID -1026
View
FAQ ID -1043
View
FAQ ID -1087
View
FAQ ID -286
View
FAQ ID -290
View
FAQ ID -2793
View
FAQ ID -2796
View
FAQ ID -372
View
FAQ ID -514
View
FAQ ID -528
View
FAQ ID -532
View
FAQ ID -619
View
FAQ ID -627
View
FAQ ID -728
View
FAQ ID -745
View
FAQ ID -813
View
-20
View
FAQ ID -2797
View
FAQ ID -1024
View
FAQ ID -2248
View
FAQ ID -1037
View
FAQ ID -337
View
FAQ ID -624
View
FAQ ID -28
View
FAQ ID -364
View
FAQ ID-3750
View
Kit Handbooks (1)
RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi
Show details
References
0
fragment fix placeholder

Customers who bought these products also bought