Organic garden soil samples were processed using MagAttract PowerSoil DNA Kit with and without the Inhibitor Removal Technology (IRT) step. Both were purified using the KingFisherTM Duo. Without the IRT step, DNA failed to amplify, while those that had been processed to remove inhibitors successfully amplified and were within the standard curve (blue lines).
Using the MagAttract PowerSoil DNA Kit on a KingFisherTM Duo, DNA was isolated from a panel of six soil samples (0.25 g). Yields varied based on the microbial load of each sample, however high-quality, high-molecular weight DNA was isolated from each sample. Replicate samples were equal in yield and quality.
Starting samples of (a) organic garden soil, (b) low biomass soil and (c) landscaping soil are known to be high in PCR inhibitors. qPCR run on 1 µl of DNA from each of these samples shows inhibitors have been removed. Undiluted and 1:10 diluted DNA samples fell within the standard curve (grey lines) with a 3-cycle difference between diluted and undiluted samples.