ipsogen NPM1 MutaScreen Kit

For detection and direct identification of NPM1 mutations type A, B, and D using real-time PCR

Features

Reliable qualitative detection
Analysis of NPM1 mutational status
Identification of mutation type (A, B, or D)
Optimal quality control and ISO 13485 certification
Easy workflow with ready-to-use solutions

Useful Resources

ipsogen NPM1 MutaScreen Handbook

Reliable detection of total and mutated NPM1.

ipsogen NPM1 MutaScreen Kit (24)

Cat. No. / ID: 677013

For 24 reactions: Wild-type NPM1 Control, Mutated NPM1 Control, Primers and Probe Mix PPM-Total NPM1, Primers and Probe Mix PPM Mutated NPM1, Primers and Probe Mix PPM-NPM1 MutA, Primers and Probe Mix PPM-NPM1 MutB, Primers and Probe Mix PPM-NPM1 MutD

For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

Product Details

The ipsogen NPM1 MutaScreen Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kit provides reagents optimized for reliable and sensitive detection allowing both qualitative analysis of NPM1 mutational status (mutated or wild-type) and identification of NPM1 mutation type (A, B or D) in genomic DNA.

Performance

To ensure accuracy, the ipsogen NPM1 MutaScreen Kit has been optimized to detect total NPM1 (wild-type and mutated), mutated NPM1, NPM1 mutations A, B, and D separately and uses DNA positive and negative controls for qualitative analysis (see figure "Reliable detection of wild-type and mutated NPM1").

Principle

The ipsogen NPM1 MutaScreen Kit is a ready-to-use kit for the detection and qualitative analysis of the mutational status of the NPM1 gene in genomic DNA (see figure "NPM1 alleles"). The kit provides reliable and reproducible results and enables detection and identification of NPM1 type A, B, and D mutations.

Procedure

The first step is extraction and determination of quality and quantity of genomic DNA. The second step is the amplification of DNA by real-time PCR. Using the ipsogen NPM1 MutaScreen Kit provides reliable detection and qualitative analysis of the mutational status of the NPM1 gene. Simply start the reaction using the optimized protocols described in the kit handbook.

Applications

The ipsogen NPM1 MutaScreen Kit enables sensitive and reliable detection and qualitative analysis of the mutational status of the NPM1 gene and identification of mutation types A, B, and D for research use only.

Supporting data and figures

Reliable detection of total and mutated NPM1.

Detection of [A] total NPM1 and [B] total NPM1, total mutated NPM1, NPM1 mut A, NPM1 mut B, and NPM1 mut D.