Formaldehyde agarose gel and corresponding northern blot (GAPDH-probed) of total RNA isolated with the QIAamp RNA Blood Mini Kit from 0.5, 1.0, and 1.5 ml (left to right) of healthy whole human blood (with indicated anticoagulants). Forty microliters of a 60 µl eluate were loaded per lane. M: 0.24–9.5 kb RNA ladder.
RT-PCR of total RNA from blood. Total RNA from 50 µl of healthy whole human blood (with indicated anticoagulants) was purified using the QIAamp RNA Blood Mini Kit and eluted with 50 µl of RNase-free water. For each sample, three RT-PCR reactions were performed (left to right) using 5 µl or 15 µl of the eluate or a control (15 µl eluate, RT omitted). For RT-PCR, samples were digested with RNase-free DNase and reverse transcription performed using an oligo-dT primer. 1/10 (2.5 µl) of the cDNA was amplified using GAPDH primers. 1/5 of the PCR reaction was loaded per lane. – PCR was performed as above but eluate was replaced with distilled water; + positive control cDNA fragment. M: 100 bp DNA ladder.