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QIAseq FastSelect –rRNA HMR Kits

For rapid rRNA and/or globin mRNA removal for RNA-seq library preparation from human, mouse, rat and other mammalian samples
  • Compatible with QIAGEN, Illumina, NEB and KAPA stranded RNA-seq library kits
  • Single reagent for human, mouse, rat and other mammalian species
  • High-performance rRNA and/or globin removal in just 14 minutes
  • Only one pipetting step – combine QIAseq FastSelect reagent with RNA and incubate
  • No extra cleanup steps or NGS library protocol changes

QIAseq FastSelect –rRNA HMR and QIAseq FastSelect –Globin Kits use a novel method to remove highly abundant RNA that is of low scientific value from your RNA-seq libraries. Researchers using RNA-seq for whole transcriptome analysis can use QIAseq FastSelect –rRNA HMR to remove cytoplasmic and mitochondrial rRNA, while researchers starting with blood samples can use QIAseq FastSelect –rRNA/Globin to remove both rRNA and globin mRNA. For researchers who are enriching for mRNA from blood, and only want to remove globin mRNA, we recommend QIAseq FastSelect –Globin. QIAseq FastSelect –rRNA HMR and QIAseq FastSelect –Globin Kits remove more unwanted RNA and are 30% faster than our previous kit versions. 

We recommend using QIAseq Stranded RNA Library Kits for robust strand-specific RNA-seq library preparation for high-quality and highly fragmented (FFPE) RNA. 

Interested in customization of a QIAseq FastSelect –rRNA HMR or QIAseq FastSelect –Globin Kit for your samples? Contact us

Want to try the QIAseq FastSelect –rRNA/Globin Kit for the first time? Request a trial kit to evaluate.

Buy Products

Cat No./ID: 334376
QIAseq FastSelect –Globin Kit (24)
Includes 3 tubes of QIAseq FastSelect reagent for globin mRNA removal; sufficient for 24 reactions from human, mouse and rat samples
Cat No./ID: 334377
QIAseq FastSelect –Globin Kit (96)

Includes 1 tube of QIAseq FastSelect reagent for globin mRNA removal; sufficient for 96 reactions from human, mouse and rat samples

Cat No./ID: 334378
QIAseq FastSelect –Globin Kit (384)

Includes 4 tubes of QIAseq FastSelect reagent for globin mRNA removal; sufficient for 384 reactions from human, mouse and rat samples

Cat No./ID: 334386
QIAseq FastSelect –rRNA HMR Kit (24)

Includes 3 tubes of QIAseq FastSelect reagent for rRNA removal; sufficient for 24 reactions from human, mouse and rat samples

Cat No./ID: 334387
QIAseq FastSelect –rRNA HMR Kit (96)

Includes 1 tube of QIAseq FastSelect reagent for rRNA removal; sufficient for 96 reactions from human, mouse and rat samples

Cat No./ID: 334388
QIAseq FastSelect –rRNA HMR Kit (384)

Includes 4 tubes of QIAseq FastSelect reagent for rRNA removal; sufficient for 384 reactions from human, mouse and rat samples

Cat No./ID: 335376
QIAseq FastSelect –rRNA/Globin Kit (24)

Includes 3 tubes of QIAseq FastSelect reagent for rRNA removal and 3 tubes of QIAseq FastSelect reagent for globin mRNA removal; sufficient for 24 reactions from human, mouse and rat samples

Cat No./ID: 335377
QIAseq FastSelect –rRNA/Globin Kit (96)

Includes 1 tube of QIAseq FastSelect reagent for rRNA removal and 1 tube of QIAseq FastSelect reagent for globin mRNA removal; sufficient for 96 reactions from human, mouse and rat samples

Cat No./ID: 335378
QIAseq FastSelect –rRNA/Globin Kit (384)

Includes 4 tubes of QIAseq FastSelect reagent for rRNA removal and 4 tubes of QIAseq FastSelect reagent for globin mRNA removal; sufficient for 384 reactions from human, mouse and rat samples

QIAseq FastSelect –rRNA HMR Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

0
QIAseq FastSelect –rRNA HMR Kit removes rRNA with 1 step in 14 minutes.
QIAseq FastSelect –rRNA HMR is a new formulation that works to remove mitochondrial and cytoplasmic rRNA from human, mouse, rat and other mammalian samples. QIAseq FastSelect HMR shows greater potency than QIAGEN’s species-specific formulations currently on the market.

A QIAseq FastSelect –rRNA HMR Kit works to remove unwanted RNAs from your RNA-seq library using 1 step. This is significantly easier than hybridization or RNase H methods, which require 29 to 37 steps (e.g., Supplier I, Supplier N and Supplier T).

B QIAseq FastSelect –rRNA HMR Kit works in only 14 minutes, which saves hours versus other methods, and is 30% faster than existing QIAseq FastSelect RNA Removal Kits.
1
QIAseq FastSelect Kit workflow.
Simply add QIAseq FastSelect reagent (rRNA Removal and/or Globin Removal) to your RNA sample, perform fragmentation (if required), then initiate a stepwise cool-down from 75°C to 25°C over 14 minutes. Researchers can then complete the remaining library preparation steps without any additional changes to their workflow. QIAseq FastSelect works with or without RNA fragmentation, providing the flexibility to use FFPE or degraded RNA samples, or high-quality RNA as part of a standard RNA-seq library construction workflow.

QIAseq FastSelect –rRNA HMR Kits, QIAseq FastSelect –Globin Kits and QIAseq FastSelect –rRNA/Globin Kit have been tested with the QIAseq Stranded Total RNA Lib Kit (QIAGEN), TruSeq Stranded (Illumina), NEBNext Ultra II Directional (New England Biolabs) and KAPA RNA HyperPrep (KAPA Biosystems) library preparation kits.
2
QIAseq FastSelect –rRNA HMR Kit is a single solution that removes mitochondrial and cytoplasmic rRNA from human, mouse, rat and other mammalian samples.
QIAseq FastSelect –rRNA HMR Kit removes more than 95% of the rRNA from human, mouse, rat, horse, cow, hamster and sheep samples and >80% removal for dog, monkey, chicken, rabbit and pig samples.
3
QIAseq FastSelect –rRNA HMR Kit has comprehensive coverage to remove cytoplasmic and mitochondrial rRNA targets.
QIAseq FastSelect –rRNA HMR was designed to remove both cytoplasmic (5S, 5.8S, 18S, 28S) and mitochondrial (12S, 16S) rRNA for human, mouse and rat samples.
4
QIAseq FastSelect –Globin Kit has comprehensive coverage to remove adult, fetal and embryonic globin mRNA.
QIAseq FastSelect –Globin was designed to remove adult, fetal and embryonic globin mRNA from human, mouse and rat samples.
5
QIAseq FastSelect –rRNA HMR Kit provides greater rRNA removal than previous kit versions.
QIAseq FastSelect –rRNA HMR is a new formulation of QIAseq FastSelect which provides a single-tube solution for human, mouse and rat samples. The new formulation shows an increased potency in removing rRNA with highly reproducible results. The improved QIAseq FastSelect –rRNA HMR Kit exhibits greater rRNA removal than kit previous versions.
6
QIAseq FastSelect –rRNA HMR Kit shows comparable gene expression results with previous kit versions.
QIAseq FastSelect –rRNA HMR Kit is a single-tube solution for human, mouse, rat and other mammalian samples. Gene expression data from human, mouse and rat universal RNA samples was compared using QIAseq FastSelect –rRNA HMR Kit and the previous versions of the kit. This data shows that the single-tube QIAseq FastSelect –rRNA HMR Kit can replace previous versions of QIAseq FastSelect RNA Removal Kits, which used species-specific human, mouse and rat solutions.
7
QIAseq FastSelect –rRNA HMR Kit provides consistent distribution of read mapping between replicates.
To test the reproducibility of the QIAseq FastSelect –rRNA HMR Kit and QIAseq Stranded Total RNA Lib Kit,100 ng of human universal RNA (HURR) was used in duplicate. Following RNA-seq library preparation and sequencing, the data was aligned using CLC Genomics Workbench. Mapped read percentages were highly reproducible, with 49% mapping to exons, 45% mapping to introns and 5% mapping to intergenic regions. Only 1.2% of the reads mapped to rRNA following treatment – a reduction of >95%.
8
QIAseq FastSelect –rRNA HMR Kit maintains the expression profile: detection of expressed genes (RPKM >0.3).
To test the reproducibility of the QIAseq FastSelect –rRNA HMR Kit and QIAseq Stranded Total RNA Lib Kit, 100 ng of human universal RNA (HURR) was used in duplicate. Following RNA-seq library preparation and sequencing, the data was aligned using CLC Genomics Workbench. An average of 14,929 genes were detected with an RPKM >0.3.
9
QIAseq FastSelect–rRNA HMR Kit maintains the expression profile: consistent detection of expressed genes (RPKM >0.3).
To test the reproducibility of the QIAseq FastSelect –rRNA HMR Kit and QIAseq Stranded Total RNA Lib Kit, 100 ng of human universal RNA (HURR) was used in duplicate. Following RNA-seq library preparation and sequencing, the data was aligned using CLC Genomics Workbench. Expressed genes with an RPKM >0.3 were compared between the 2 replicates resulting in a high degree of concordance.
10
QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly efficient and reproducible removal of rRNA and globin mRNA, regardless of RNA input.
Stranded transcriptome libraries were prepared from 100 ng and 1 µg aliquots of human blood total RNA using the QIAseq FastSelect –rRNA/Globin Kit and QIAseq Stranded Total RNA Lib Kit.

To remove rRNA and globin mRNA during the QIAseq library prep, QIAseq FastSelect –rRNA HMR + –Globin was combined with each RNA sample and 5x RT Buffer. The samples were then heated (fragmented) at 95°C for 15 min and stepwise cooled to 25°C over 14 minutes. The remaining library prep steps were then performed according to the QIAseq Stranded Total RNA Lib Kit instructions. Sequencing was performed on a NextSeq 550 and data analysis was performed using CLC Biomedical Workbench. The percent or reads mapping to rRNA and globin is shown.
11
QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly consistent breakdown of mapped reads, regardless of RNA input.
Stranded transcriptome libraries were prepared from 100 ng and 1 µg aliquots of human blood total RNA using the QIAseq FastSelect –rRNA/Globin Kit and QIAseq Stranded Total RNA Lib Kit.  

To remove rRNA and globin mRNA during the QIAseq library prep, QIAseq FastSelect –rRNA HMR + –Globin was combined with each RNA sample and 5x RT Buffer. The samples were then heated (fragmented) at 95°C for 15 min and stepwise cooled to 25°C over14 minutes. The remaining library prep steps were then performed according to the QIAseq Stranded Total RNA Lib Kit instructions. Sequencing was performed on a NextSeq 550 and data analysis was performed using CLC Biomedical Workbench. Mapped read percentages were highly reproducible with 35% mapping to exon, 58% mapping to introns and 5% mapping to intergenic regions. Only 1–2% of the reads mapped to rRNA and less than 1% mapped to globin transcripts.
12
QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: gene expression results.
Stranded transcriptome libraries were prepared from 100 ng and 1 µg aliquots of human blood total RNA using the QIAseq FastSelect –rRNA/Globin Kit and QIAseq Stranded Total RNA Lib Kit.

To remove rRNA and globin mRNA during the QIAseq library prep, QIAseq FastSelect –rRNA HMR + –Globin was combined with each RNA sample and 5x RT Buffer. The samples were then heated (fragmented) at 95°C for 15 min and stepwise cooled to 25°C over 14 minutes. The remaining library prep steps were then performed according to the QIAseq Stranded Total RNA Lib Kit instructions. Sequencing was performed on a NextSeq 550 and data analysis was performed using CLC Biomedical Workbench. Gene expression results for 100 ng and 1 µg of RNA, for genes with an RPKM >0.3, show a high degree of correlation.
13
QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples.
QIAseq FastSelect –rRNA HMR Kit removes rRNA from FFPE RNA fusion gene reference samples.

FFPE RNA reference standards A FFPE Horizon 5-Fusion Multiplex Positive and B FFPE Horizon 5-Fusion Multiplex Negative Control were used to prepare duplicate RNA-seq libraries using QIAseq FastSelect –rRNA HMR and QIAseq Stranded Total RNA Lib Kits.  QIAseq FastSelect consistently removed >95% of the rRNA from the sample even though the input RNA was highly fragmented.
14
QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: removal of rRNA leads to increased exon mapping.
QIAseq FastSelect –rRNA HMR Kit removes rRNA from FFPE RNA fusion gene reference samples.

FFPE RNA reference standards A FFPE Horizon 5-Fusion Multiplex Positive and B FFPE Horizon 5-Fusion Multiplex Negative Control were used to prepare duplicate RNA-seq libraries using QIAseq FastSelect –rRNA HMR and QIAseq Stranded Total RNA Lib Kits. Removal of rRNA leads to an increase in exon mapping. FFPE Horizon 5-Fusion Multiplex Positive and Negative Control samples show an exon mapping rate of approximately 40% versus 10% in the untreated samples.
15
QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: gene expression results.
QIAseq FastSelect –rRNA HMR Kit removes rRNA from FFPE RNA fusion gene reference samples.

When rRNA depletion is performed using QIAseq FastSelect (Log2 normalized gene expression, targets with RPKM >0.3), gene expression results are highly reproducible.
16
QIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samples.
QIAseq FastSelect –rRNA HRM removes rRNA from FFPE RNA samples.

A QIAseq FastSelect results in highly efficient removal of rRNA from fragmented samples. The percentage of reads mapped to rRNA is shown. B Exon/intron/intergenic read mapping is shown. QIAseq FastSelect results in a substantial increase in the percentage of reads mapped to exonic regions.
17
QIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samples: gene expression results.
QIAseq FastSelect –rRNA HRM removes rRNA from FFPE RNA samples.

When rRNA depletion is performed using QIAseq FastSelect (Log2 normalized gene expression, targets with RPKM >0.3), QIAseq FastSelect treatment vs. no treatment results suggest that QIAseq FastSelect does not perturb the natural expression profile of samples.
Performance

QIAseq FastSelect –rRNA HMR Kits demonstrated highly efficient removal of rRNA and superior performance compared to our previous kit versions and alternative products.
See figures:
QIAseq FastSelect –rRNA HMR Kit removes rRNA with 1 step in 14 minutes
QIAseq FastSelect –rRNA HMR Kit has comprehensive coverage to remove cytoplasmic and mitochondrial rRNA targets
QIAseq FastSelect –Globin Kit has comprehensive coverage to remove adult, fetal and embryonic globin mRNA RNA
QIAseq FastSelect –rRNA HMR Kit provides greater rRNA removal than previous kit versions
QIAseq FastSelect –rRNA HMR Kit shows comparable gene expression results with previous kit versions
QIAseq FastSelect –rRNA HMR Kit maintains the expression profile: detection of expressed genes (RPKM >0.3)
QIAseq FastSelect–rRNA HMR Kit maintains the expression profile: consistent detection of expressed genes (RPKM >0.3)

Robust performance with a variety of sample types, regardless of RNA input

QIAseq FastSelect provides reliable rRNA removal, consistent read mapping and reproducible gene expression results with RNA extracted from various sample types, including FFPE, and over a broad range of RNA input amounts.
See figures:
QIAseq FastSelect –rRNA HMR Kit is a single solution that removes mitochondrial and cytoplasmic rRNA from human, mouse, rat and other mammalian samples
QIAseq FastSelect –rRNA HMR Kit provides consistent distribution of read mapping between replicates
QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly efficient and reproducible removal of rRNA and globin mRNA, regardless of RNA input
QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: highly consistent breakdown of mapped reads, regardless of RNA input
QIAseq FastSelect –rRNA/Globin Kit shows consistent results over a broad range of RNA input: gene expression results
QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples
QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: removal of rRNA leads to increased exon mapping
QIAseq FastSelect –rRNA HMR works with FFPE and degraded RNA samples: gene expression results
QIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samples
QIAseq FastSelect –rRNA HMR: robust performance with FFPE RNA samples: gene expression results

Principle

Most RNA removal or depletion strategies associated with RNA-seq library construction are sample pre-treatment strategies involving hybrid-capture or enzymatic removal of unwanted RNA. Our unique QIAseq FastSelect procedure is compatible with QIAGEN, Illumina, KAPA and NEB stranded library preparation kits and provides complete rRNA removal in a single, 14-minute inline step. This is dramatically faster than alternative RNA depletion kits, which require pre-treatment protocols involving more than 25 steps and 2 hours to complete. 

Simply add QIAseq FastSelect reagent (rRNA Removal and/or Globin Removal) to the RNA sample, perform fragmentation (if required), stepwise cool the reaction from 75°C to 25°C for 14 minutes and then complete the remaining library preparation steps. QIAseq FastSelect works with or without RNA fragmentation, providing the flexibility to use RNA from FFPE samples or degraded RNA samples, or high-quality RNA as part of a standard RNA-seq library construction workflow.

Procedure

Most RNA removal or depletion strategies associated with RNA-seq library construction are sample pre-treatment strategies involving hybrid-capture or enzymatic removal of unwanted RNA. Our unique QIAseq FastSelect procedure is compatible with QIAGEN, Illumina, KAPA and NEB stranded library preparation kits and provides complete rRNA removal in a single, 14-minute inline step (figure QIAseq FastSelect Kit workflow). This is dramatically faster than alternative RNA depletion kits, which require pre-treatment protocols involving more than 25 steps and 2 hours to complete. 

Simply add QIAseq FastSelect reagent (rRNA Removal and/or Globin Removal) to the RNA sample, perform fragmentation (if required), stepwise cool the reaction from 75°C to 25°C for 14 minutes and then complete the remaining library preparation steps. QIAseq FastSelect works with or without RNA fragmentation, providing the flexibility to use RNA from FFPE samples or degraded RNA samples, or high-quality RNA as part of a standard RNA-seq library construction workflow.

Applications

QIAseq FastSelect delivers rapid, reliable RNA removal from FFPE and fresh sample RNA sources. QIAseq FastSelect –rRNA HMR and QIAseq FastSelect –Globin Kits are available in a variety of different formats and sizes to suit your specific applications. 

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