The miRCURY LNA miRNA ISH Optimization Kit 1 was used to detect miR-1 in mouse heart FFPE tissue. Staining was performed with NBT-BCIP (blue). Sections were counterstained with Nuclear Red.
The miRCURY LNA miRNA ISH Optimization Kit 2 was used to detect miR-21 in colon adenocarcinoma FFPE tissue. Staining was performed with NBT-BCIP (blue). Sections were counterstained with Nuclear Red.
The miRCURY LNA miRNA ISH Optimization Kit 3 was used to detect miR-122 in normal murine liver FFPE tissue. Staining was performed with NBT-BCIP (blue). Sections were counterstained with Nuclear Red.
The miRCURY LNA miRNA ISH Optimization Kit 4 was used to detect miR-124 in human brain lesion FFPE tissue. Staining was performed with NBT-BCIP (blue). Sections were counterstained with Nuclear Red.
The miRCURY LNA miRNA ISH Optimization Kit 5 was used to detect miR-126 in FFPE samples with inflamed colon tissue. Staining was performed with NBT-BCIP (blue). Sections were counterstained with Nuclear Red.
The miRCURY LNA miRNA ISH Optimization Kit 7 was used to detect miR-145 in FFPE tissue of human colon wall with underlying muscle layers. Staining was performed with NBT-BCIP (blue). Sections were counterstained with Nuclear Red.
The miRCURY LNA miRNA ISH Optimization Kit 8 was used to detect miR-205 in human breast carcinoma FFPE tissue. Staining was performed with NBT-BCIP (blue). Sections were counterstained with Nuclear Red.
The miRCURY LNA miRNA ISH Optimization Kit 9 was used to detect miR-223 in FFPE samples with human esophagus cancer. Staining was performed with NBT-BCIP (blue). Sections were counterstained with Nuclear Red.
First, the tissue is "opened" using Protease K. In the hybridization step, the DIG-labeled LNA probe binds specifically to its target miRNA. Alkaline phosphatase (AP)-conjugated anti-DIG antibodies are then added. This step is followed by NBT-BCIP development and optional counterstaining with Nuclear Red.
Choosing the appropriate miRCURY LNA miRNA ISH Optimization Kit. The table indicates the tissue(s) in which each of the kits has been validated.
