Attractene Transfection Reagent
For efficient DNA transfection and DNA–siRNA/miRNA cotransfection
Attractene Transfection Reagent represents the next generation in lipid technology, ensuring highly efficient DNA transfection of eukaryotic cells. Attractene Reagent is a nonliposomal lipid that enables transfection of all adherent cells, including difficult-to-transfect cell types such as HaCaT, MonoMac6, and HCT116, and some suspension cell types (Jurkat, K562). It is also highly suitable for cotransfection of DNA with siRNA or miRNA mimics or inhibitors.
Attractene Reagent provided higher transfection efficiency than alternative reagents when transfection was performed without any optimization following the recommended conditions provided in the handbooks (see figure "Attractene Reagent outperforms alternative reagents"). Cytotoxicity must be kept to a minimum to ensure that results are due to the nucleic acid transfected and not the transfection process itself. Attractene Reagent ensures exceptionally low cytotoxicity (see figure "Healthy cells after transfection using Attractene Reagent"). Using Attractene Reagent ensures that transfection of shRNA (short-hairpin RNA) vectors for gene silencing experiments can be achieved with high efficiency (see figure "Effective knockdown after shRNA vector transfection").
Attractene Reagent is a nonliposomal lipid which enables highly efficient DNA transfection of some suspension cell types (Jurkat, K562), and adherent cells, including difficult-to-transfect cell types such as HaCaT, MonoMac6, and HCT116. Attractene Reagent consistently provides higher transfection efficiency than alternative reagents under a range of experimental conditions. This feature allows flexible handling and ease of use for many experimental setups such as using automated platforms and transfection at high- or low-throughput levels. It is also highly suitable for cotransfection of DNA with siRNA or miRNA mimics or inhibitors. Using Attractene Reagent ensures exceptionally low cytotoxicity, which is critical to successful transfection experiments.
Attractene Transfection Reagent is highly suited for rapid fast-forward DNA transfection. In Fast-Forward Protocols, cells are seeded and transfected on the same day (see figure "Flexible, rapid Fast-Forward Protocols"). This is quicker, saves labor, and increases experimental flexibility compared to protocols where cells are seeded the day before transfection.
Cell type-specific protocols at the TransFect Protocol Database
In addition to the protocols provided in the Attractene Transfection Reagent Handbook, you can find protocols to suit your cell type and plate/dish format using QIAGEN's TransFect Protocol Database. The database provides exactly the protocol needed, saving time and effort. Simply enter the cell type and nucleic acid of interest to receive a QIAGEN transfection protocol to print out or download in convenient PDF format. Use of the TransFect Protocol Database is free of charge and no registration is required.
Attractene Transfection Reagent can be used for a range of transfection applications, including transient or stable transfection, or cotransfection of multiple DNA constructs, and cotransfection of DNA with siRNA or miRNA mimics or inhibitors. Attractene Transfection Reagent can be used in:
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