Total RNA from various [A] human, [B] mouse, [C] rat, and [D] dog tissues was used for expression analysis of snoRNA/snRNA using 6 miScript PCR Controls. CT values show that the expression level of each RNA was very similar in different tissue types.
Wells H3 to H8 each contain an assay for a different snoRNA/snRNA that can be used as a normalization control for the array data (SN1=SNORD61 assay, SN2=SNORD68 assay, SN3=SNORD72 assay, SN4=SNORD95 assay, SN5=SNORD96A assay, SN6=RNU6B/RNU6-2 assay). Wells H1 and H2 contain replicate C. elegans miR-39 miScript Primer Assays that can be used as an alternative normalizer for array data (Ce). Wells H9 and H10 contain replicate reverse transcription controls (miRTC). Wells H11 and H12 contain replicate positive PCR controls (PPC). These controls are also included in 384-well plate and Rotor-Disc 100 format miScript miRNA PCR Arrays.