RT² SYBR^® Green qPCR Mastermixes

For use with RT² Profiler, RT² lncRNA, EpiTect ChIP, and EpiTect Methyl II PCR Arrays, and qPCR Assays

Designed exclusively for use with RT²and EpiTect Methyl II qPCR Assays
Delivers specific amplification with minimal primer-dimers
Available with ROX, fluorescein, or without dye
For use with qPCR instruments under standard cycling conditions

RT² SYBR Green qPCR Mastermixes are highly suited for real-time PCR applications using SYBR Green, and are required for guaranteed qPCR performance of the RT² qPCR Primer Assays, RT² Profiler PCR Arrays, RT² lncRNA qPCR Assays, and RT² lncRNA PCR Arrays. This Mastermix should be used in qPCR instruments under standard cycling conditions. RT² SYBR Green qPCR Mastermixes are also suitable for use with EpiTect ChIP and EpiTect Methyl II qPCR Assays and PCR Arrays.

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Cat No./ID: 330500

RT² SYBR Green qPCR Mastermix (2)

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Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Arrays or 200 x 25 µl reactions or 400 x 10 µl reactions

Cat No./ID: 330501

RT² SYBR Green qPCR Mastermix (8)

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Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactions or 2000 x 10 µl reactions

Cat No./ID: 330502

RT² SYBR Green qPCR Mastermix (12)

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Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactions or 3000 x 10 µl reactions

Cat No./ID: 330503

RT² SYBR Green qPCR Mastermix (24)

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Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x 10 µl reactions

Cat No./ID: 330504

RT² SYBR Green qPCR Mastermix (6)

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Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Arrays or 600 x 25 µl reactions or 1500 x 10 µl reactions

Cat No./ID: 330509

RT² SYBR Green qPCR Mastermix (25 ml)

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Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactions or 5000 x 10 µl reactions

Cat No./ID: 330510

RT² SYBR Green Fluor qPCR Mastermix (2)

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Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Array or 200 x 25 µl reactions or 400 x 10 µl reactions

Cat No./ID: 330511

RT² SYBR Green Fluor qPCR Mastermix (8)

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Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactions or 2000 x 10 µl reactions

Cat No./ID: 330512

RT² SYBR Green Fluor qPCR Mastermix (12)

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Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactions or 3000 x 10 µl reactions

Cat No./ID: 330513

RT² SYBR Green Fluor qPCR Mastermix (24)

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Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x10 µl reactions

Cat No./ID: 330514

RT² SYBR Green Fluor qPCR Mastermix (6)

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Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Array or 600 x 25 µl reactions or 1500 x 10 µl reactions

Cat No./ID: 330519

RT² SYBR Green Fluor qPCR Mastermix (25 ml)

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Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactions or 5000 x 10 µl reactions

Cat No./ID: 330520

RT² SYBR Green ROX qPCR Mastermix (2)

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Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Array or 200 x 25 µl reactions or 400 x 10 µl reactions

Cat No./ID: 330521

RT² SYBR Green ROX qPCR Mastermix (8)

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Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactions or 2000 x 10 µl reactions

Cat No./ID: 330522

RT² SYBR Green ROX qPCR Mastermix (12)

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Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactions or 3000 x 10 µl reactions

Cat No./ID: 330523

RT² SYBR Green ROX qPCR Mastermix (24)

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Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x 10 µl reactions

Cat No./ID: 330524

RT² SYBR Green ROX qPCR Mastermix (6)

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Contains 6 x 1.35 ml tubes: for 6 x 96-well RT² PCR Arrays, 3 x 384-well RT² PCR Array or 600 x 25 µl reactions or 1500 x 10 µl reactions

Cat No./ID: 330529

RT² SYBR Green ROX qPCR Mastermix (25 ml)

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Contains 1 bottle of 25 ml mastermix: for 20 x 96-well RT² PCR Arrays, 12 x 384-well RT² PCR Arrays or 2000 x 25 µl reactions or 5000 x 10 µl reactions

RT² SYBR^® Green qPCR Mastermixes are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

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High amplification efficiency over a wide dynamic range.

Using duplicate 10-fold serial dilutions of purified template and the RT² qPCR Primer Assay for the Human Nicotinic Acetylcholine Receptor Alpha 5 (CHRNA5), RT² SYBR^® Green Mastermixes demonstrate a 6-log linear dynamic range.

Tighter control of polymerase activity yields greater specificity.

Greater specificity was achieved using RT² SYBR^® Green Mastermixes [A] compared to master mixes from an alternative supplier [B].

Performance

RT² SYBR Green qPCR Mastermixes contain all of the optimized reagents and buffers needed for SYBR Green based real-time PCR with RT² qPCR Primer Assays, RT² Profiler PCR Arrays, RT² lncRNA qPCR Assays, and RT² lncRNA PCR Arrays on all real-time PCR instruments.

The high-performance real-time PCR enabled by RT² SYBR Green qPCR Mastermixes demonstrates high amplification efficiencies (see figure "High amplification efficiency over a wide dynamic range") and high levels of sensitivity and specificity (see figure "Tighter control of polymerase activity yields greater specificity").

Principle

RT² SYBR Green qPCR Mastermixes contain real-time PCR buffer, a high-performance, HotStart DNA Taq polymerase, nucleotides, and SYBR Green dye. Some mastermixes contain either fluorescein or ROX dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate SYBR Green results by preventing the amplification of primer–dimers and other nonspecific products.

Mastermixes without ROX or fluorescein

RT² SYBR Green qPCR Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation not requiring a reference dye, including Bio-Rad models CFX96, CFX384; Bio-Rad/MJ Research Chromo4, DNA Engine Opticon, DNA Engine Opticon 2; Roche LightCycler 480 (96-well and 384-well); Eppendorf Mastercycler ep realplex without ROX filter set; and Cepheid SmartCycler.

Mastermixes with fluorescein

RT² SYBR Green Fluor qPCR Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation that uses fluorescein as a reference dye, including the Bio-Rad models iCycler, iQ5, MyiQ, and MyiQ2.

Mastermixes with ROX

RT² SYBR Green ROX qPCR Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation that uses ROX as a reference dye, including QIAGEN’s Rotor-Gene Q; Applied Biosystems models 5700, 7000, 7300, 7500 (Standard and Fast), 7700, 7900HT (Standard and Fast 96-well block, 384-well block), StepOnePlus, ViiA 7 (Standard and Fast 96-well block, 384-well block); Eppendorf Mastercycler ep realplex with or without ROX filter set; Stratagene models Mx3000P, Mx3005P, Mx4000; and Takara TP-800.

Applications

RT² SYBR Green qPCR Mastermixes are suitable for use in real-time PCR applications, especially RT² qPCR Primer Assays, RT² Profiler PCR Arrays, RT² lncRNA qPCR Assays, and RT² lncRNA PCR Arrays.

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FAQs (16)

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	Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays? FAQ ID -2675	View
	How does HotStart PCR help minimize nonspecific amplification events? FAQ ID -2676	View
	What is a dissociation curve, and why is it important to run a dissociation curve, following qPCR using SYBR Green chemistry? FAQ ID -2678	View
	How can I ensure that reaction volume is not lost due to evaporation during thermal cycling? FAQ ID -2679	View
	Are primers available that only detect mitochondrial DNA encoded genes and not nuclear genomic DNA encoded genes? FAQ ID -2680	View
	What is the delta Rn value? FAQ ID -2681	View
	What is the threshold cycle or Ct value? FAQ ID -2682	View
	Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay? FAQ ID -2684	View
	Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay? FAQ ID -2685	View
	How can I predict the percent qPCR signal due to contaminating DNA, for a given qPCR assay, and its matching NRT control? FAQ ID -2688	View
	Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays? FAQ ID -2706	View
	What do I need to complete a RT² qPCR Primer Assay? FAQ ID -2707	View
	Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays? FAQ ID -2713	View
	Which qPCR instrument should I use with your RT² qPCR Primer Assays? FAQ ID -2714	View
	What would happen if I used home-made PCR master mixes or master mixes from other manufacturers with the RT² products? FAQ ID -2715	View
	Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be? FAQ ID -2717	View

Instrument Technical Documents (1)

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	(EN) - RT2 Profiler PCR Arrays For pathway-focused gene expression analysis	Show details

Kit Handbooks (1)

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	RT2 Profiler PCR Array Handbook (Nov2018) For pathway-focused gene expression profiling using real-time RT-PCR	Show details

Application Notes (1)

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	(EN) - Purification of total RNA from peripheral blood mononuclear cells	Show details

Safety Data Sheets (18)

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	MSDS RT² SYBR Green Fluor qPCR Mastermix (12)
	MSDS RT² SYBR Green Fluor qPCR Mastermix (6)
	MSDS RT² SYBR Green Fluor qPCR Mastermix (2)
	MSDS RT² SYBR Green Fluor qPCR Mastermix (24)
	MSDS RT² SYBR Green Fluor qPCR Mastermix (25 ml)
	MSDS RT² SYBR Green Fluor qPCR Mastermix (8)
	MSDS RT² SYBR Green qPCR Mastermix (12)
	MSDS RT² SYBR Green qPCR Mastermix (2)
	MSDS RT² SYBR Green qPCR Mastermix (24)
	MSDS RT² SYBR Green qPCR Mastermix (25 ml)
	MSDS RT² SYBR Green qPCR Mastermix (6)
	MSDS RT² SYBR Green qPCR Mastermix (8)
	MSDS RT² SYBR Green ROX qPCR Mastermix (12)
	MSDS RT² SYBR Green ROX qPCR Mastermix (2)
	MSDS RT² SYBR Green ROX qPCR Mastermix (24)
	MSDS RT² SYBR Green ROX qPCR Mastermix (25 ml)
	MSDS RT² SYBR Green ROX qPCR Mastermix (8)
	MSDS RT² SYBR Green ROX qPCR Mastermix (6)

Product citations in scientific articles

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RT2 SYBR® Green qPCR Mastermixes

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Product Details

Mastermixes without ROX or fluorescein

Mastermixes with fluorescein

Mastermixes with ROX

Product Resources

Product citations in scientific articles

RT² SYBR^® Green qPCR Mastermixes