QIAamp Investigator BioRobot Kit

For automated medium- to high-throughput purification of forensic DNA on the BioRobot Universal System

Features

  • DNA purification from forensic and human identity samples
  • More efficient yields from trace casework samples
  • Higher signal-to-noise ratios
  • Rapid isolation of high-quality, ready-to-use DNA

Useful Resources

QIAamp Investigator BioRobot Kit (12)

Cat. No. / ID: 965942

For 12 x 96 DNA preps: QIAamp 96 Plates, S-Blocks, Buffers, Proteinase K, Elution Microtubes CL, Caps, Disposable Troughs
The QIAamp Investigator BioRobot Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The QIAamp Investigator BioRobot Kit provides automated DNA purification from up to 96 samples (such as filters, casework or crime-scene samples, and blood) or up to 192 swabs on the BioRobot Universal System using proven QIAamp silica-membrane technology. The fully automated procedure on the BioRobot Universal System requires less than 3 hours, including bar code reading, sample tracking and complete process documentation, with no hands-on time during the run. The purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios.

Performance

The QIAamp Investigator BioRobot Kit is suited for many applications, such as:

  • Purification of DNA from forensic casework and database samples
  • DNA purification from different trace samples (e.g., crime-scene)
  • Routine analysis of reference samples
Efficient yields from trace casework samples

The automated procedure is highly efficient, providing high yields of DNA (see figure " Superior DNA yields"). The purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis (see figure " High-quality STR profiles"), with high signal-to-noise ratios. The kit provides high-quality DNA from 24 to 96 forensic samples in batches of 8 (see figure " High-quality DNA from a wide range of sample types").

See figures

Principle

The BioRobot Universal System together with the Application Pack, Investigator and the QIAamp Investigator Kit meet the demands of forensic laboratories with flexible, fully automated, standardized processing using proven QIAamp chemistry.

Procedure

The robust purification procedure provides high process safety and helps to prevent cross-contamination. For increased convenience and flexibility, there is the option of walkaway sample lysis and DNA purification or, if required, lysis can be performed manually followed by walkaway purification of DNA. The fully automated procedure on the BioRobot Universal System requires less than 3 hours, including bar code reading, sample tracking and complete process documentation, with no hands-on time during the run.

Applications

The purification procedure of the QIAamp Investigator BioRobot Kit provides high-quality DNA from 24 to 96 forensic samples in batches of 8. Using the kit, the following samples can be processed together in a single run:

  • Dried blood
  • Surface swabs (e.g., blood, semen, saliva, and epithelial cells)
  • Stains on fabric (e.g., blood- or saliva-stained fabrics)
  • Cigarette butts and tobacco
  • Chewing gum and envelopes

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsSTR Typing, PCR, blotting
Time per run or per prep<3 hours
Yield1-2 µg DNA per swab; varying for casework samples
For automated processingBiorobot Universal platform
ProcessingAutomated
Sample amount1 swab per well, up to 30 mg chewing gum, up to 40 mg cigarette butts, envelops, fabric or hair
Main sample typeForensic casework and human identity samples
Elution volume150 µl
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinGenomic DNA
Format96-well plate
TechnologySilica technology
Samples per run (throughput)24-96 samples per run

Resources

Kit Handbooks (1)
For automated purification of DNA from forensic casework and human identity samples using the BioRobot Universal System

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728