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ipsogen MPL W515L/K MutaScreen Kit

For detection of MPL W515L and MPL W515K mutations using real-time PCR
  • Reliable qualitative detection
  • Multiplex analysis with no need for optimization
  • Optimal quality control and ISO 13485 certification
  • Easy workflow with ready-to-use solutions
The ipsogen MPL W515L/K MutaScreen Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The ipsogen MPL W515L/K MutaScreen Kit provides reagents optimized for reliable and sensitive detection and qualitative analysis of the MPL W515L and MPL W515K mutations in human genomic DNA.
Cat No./ID: 676413
ipsogen MPL W515L/K MutaScreen Kit (24)
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For 24 reactions: MPL W515L Positive Control, MPL W515K Positive Control, MPL Negative Control, MPL W515L Cut-Off Sample, MPL W515K Cut-Off Sample, Primers and Probes Mix MPL Wild-Type and MPL W515L, Primers and Probes Mix MPL Wild-Type and MPL W515K
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MPL mutations.
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Representative allelic discrimination.
Scatter plots of representative allelic discrimination experiments with cut-off samples (COS), DNA positive controls (PC), and negative control (NC) for qualitative detection of [A] the MPL W515L mutation and [B] the MPL W515K mutation in genomic DNA.
Performance
To ensure accuracy, the ipsogen MPL W515L/K MutaScreen Kit has been optimized to detect the MPL W515L and W515K mutations and uses DNA positive and negative controls for qualitative analysis (see figure "Representative allelic discrimination"). The ipsogen MPL W515L/K MutaScreen Kit provides DNA cut-off samples (COS) corresponding to 1.5% of MPL W515K and 1.5% of MPL W515L, ensuring accurate qualitative analysis.
Principle
The ipsogen MPL W515L/K MutaScreen Kit is a ready-to-use kit for the detection and qualitative analysis of the MPL W515L and W515K mutations independently in human genomic DNA (see figure "MPL mutations"). The kit provides reliable and reproducible results. The ipsogen MPL W515L/K MutaScreen Kit provides DNA cut-off samples (COS) for MPL W515L and MPL W515K mutations, DNA positive controls (PC), and a negative control (NC) for MPL W515L and MPL W515K. Use of the cut-off samples and controls enables accurate qualitative analysis of genotyping results.
Procedure
The first step is extraction and determination of quality and quantity of genomic DNA. The second step is the amplification of DNA by real-time PCR. Using the ipsogen MPL W515L/K MutaScreen Kit provides reliable detection and qualitative analysis of the MPL W515L and W515K mutations independently. Simply start the reaction using the optimized protocols described in the kit handbook.
Applications
The ipsogen MPL W515L/K MutaScreen Kit enables sensitive and reliable detection, qualitative, or semi-quantitative analysis of the MPL W515L and MPL W515K mutations for research use only.

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