ipsogen CBFB-MYH11 A KitFor quantitative detection of CBFB-MYH11 A fusion gene transcripts using real-time PCR
The ipsogen CBFB-MYH11 A Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The ipsogen CBFB-MYH11 A Kit provides reagents optimized for reliable and sensitive detection and quantification of CBFB-MYH11 A fusion gene transcripts in bone marrow or peripheral blood samples, relative to ABL control gene expression.
Buy Products
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
To ensure highest sensitivity, the ipsogen CBFB-MYH11 A Kit has been optimized to detect ABL and CBFB-MYH11 A fusion gene transcripts over a wide dynamic range (see figure "Sensitive detection of CBFB-MYH11 A and ABL standards").
Principle
The ipsogen CBFB-MYH11 A Kit is a ready-to-use kit for detection and quantification of CBFB-MYH11 A fusion gene transcripts using real-time PCR. The kit is based on the amplification and detection of specific CBFB-MYH11 A fusion gene transcripts, relative to ABL control gene expression in total RNA (see figures "CBFB-MYH11 A fusion gene transcript" and "ABL control gene transcript", and provides high levels of specificity, sensitivity, and reproducibility.
Each ipsogen CBFB-MYH11 A Kit provides 3 standard dilutions for the ABL gene and 5 standard dilutions for the CBFB-MYH11 A fusion gene. Primers and probe sets are designed according to Europe Against Cancer (EAC) recommendations.
Procedure
The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using the ipsogen CBFB-MYH11 A Kit allows detection and quantification of CBFB-MYH11 A fusion gene and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.
Applications
The ipsogen CBFB-MYH11 A Kit enables sensitive and reliable detection and quantification of CBFB-MYH11 A fusion gene transcripts relative to ABL control gene expression for research use only.
Product Resources |