Reliable detection of miRNAs.
Reliable detection of miRNAs.
Efficient disruption of animal tissues.
Efficient disruption of animal tissues.
Reproducible RNA purification from plant tissues.
Reproducible RNA purification from plant tissues.
Intact protein suitable for all types of analysis.
Intact protein suitable for all types of analysis.
High-quality DNA from animal tissues.
High-quality DNA from animal tissues.
High-quality DNA from plant tissues.
High-quality DNA from plant tissues.
TissueLyser II.
TissueLyser II.
Reliable detection of miRNAs. Rat kidney was stabilized in RNAlater RNA Stabilization Reagent, and 25 mg samples were disrupted in QIAzol Lysis Reagent for 2 x 5 minutes at 25 Hz using the TissueLyser II. Total RNA containing miRNA was purified using the miRNeasy Mini Kit, using either the manual procedure (Manual) or the automated procedure with the QIAcube (QIAcube). Real-time RT-PCR using the miScript System was carried out to detect 2 different miRNAs, miR-16 and miR-25.
Efficient disruption of animal tissues. Tissues (20 mg) were either frozen (Frozen) or stabilized in RNAlater RNA Stabilization Reagent (RNAlater), and then disrupted using the TissueLyser II (2 x 2 minutes for liver and muscle; 2 x 5 minutes for skin). RNA was purified using the RNeasy Mini Kit (liver), RNeasy Fibrous Tissue Mini Kit (skin), or RNeasy Lipid Tissue Mini Kit (muscle). [A] Analysis on a 1.2 % formaldehyde agarose gel shows sharp ribosomal RNA bands, indicating intact RNA. [B] RNA yields were quantified by A260 nm absorbance measurements.
Reproducible RNA purification from plant tissues. Frozen plant leaves were disrupted using the TissueLyser II (2 x 1 minute). RNA was purified using the RNeasy Plant Mini Kit and analyzed on a 1.2 % formaldehyde agarose gel. The ribosomal RNA bands were sharp and of equal intensity, indicating reproducible purification of intact RNA. T: tomato (100 mg); A: arabidopsis (25 mg); C: cotton (100 mg); M: maize (100 mg); R: rape (100 mg).
Intact protein suitable for all types of analysis. Various rat tissues (25 mg each) stabilized in Allprotect Tissue Reagent were disrupted using the TissueLyser LT or TissueLyser II. Total protein was purified using the Qproteome Mammalian Protein Prep Kit and then analyzed by western blotting. Transfer membrane stained with Ponceau S, and western blot. LT: TissueLyser LT; II: TissueLyser II; M: markers.
High-quality DNA from animal tissues. Various rat tissues (25 mg each) stabilized in Allprotect Tissue Reagent were disrupted using the TissueLyser LT or TissueLyser II. DNA was purified on the QIAcube using the DNeasy Blood & Tissue Kit, and then used in PCR with the HotStarTaq Plus Master Mix Kit and a PGK1 primer system. The 120 bp PCR product was analyzed on the QIAxcel using the QIAxcel DNA High Resolution Kit. LT: TissueLyser LT; II: TissueLyser II; M: markers.
High-quality DNA from plant tissues. Various plant tissues (100 mg each) were disrupted in precooled adapters using the TissueLyser LT or TissueLyser II. DNA was purified on on the QIAcube using the DNeasy Plant Mini Kit and then analyzed on an agarose gel. LT: TissueLyser LT; II: TissueLyser II; M: markers.
TissueLyser II.