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MagAttract DNA Blood Mini M48 Kit

For automated purification of genomic DNA from 100–200 µl blood samples
  • Rapid purification of up to 6 µg high-quality DNA
  • Flexible purification of 6–48 samples per run
  • Easy optimization of DNA concentration

The MagAttract DNA Blood Mini M48 Kit provides automated purification of genomic DNA on the BioRobot M48 using proven MagAttract magnetic particle technology. DNA purification and magnetic separation take place in the pipet tips, increasing the efficiency of the procedure. Up to 48 samples can be processed per run in increments of 6 samples. Variable elution volumes enable easy optimization of DNA concentration.

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Cat No./ID: 951336
MagAttract DNA Blood Mini M48 Kit (192)
£517.00
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MagAttract Suspension B, Buffers, and RNase-Free Water for up to 192 x 200 µl preps
The MagAttract DNA Blood Mini M48 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Performance
The MagAttract DNA Blood Mini M48 Kit provides fully automated purification of DNA (see figure "Reproducible purification of high-quality DNA") and the purified DNA is free of PCR inhibitors (see figure "Reproducible performance in sensitive PCR analysis"). The MagAttract DNA Blood Midi M48 Kit is suitable for use with ACD-, heparin-, and EDTA-preserved whole blood samples (see figure "High-quality genomic DNA from differently preserved whole-blood samples").
Principle

The MagAttract DNA Blood Mini M48 Kit is designed for fully automated DNA purification. Proven MagAttract magnetic particle technology in combination with the BioRobot M48 workstation provides high-quality DNA, suitable for direct use in sensitive downstream applications. Advantages of the system include scalable sample volumes and elution volumes (see table ), giving the yield and concentration of high-quality DNA appropriate for the intended downstream application.  

Sample and elution volumes for the MagAttract DNA Blood M48 procedure
SampleKitProtocolAmount of starting material (µl)Elution volume (µl)
BloodMagAttract DNA Blood Mini M48 Kit200 µl Blood100–20050–400
BloodMagAttract DNA Blood Midi M48 Kit350 µl Blood250–350100–400
BloodMagAttract DNA Blood Midi M48 Kit700 μl Blood 500–700200–400
Buffy coat enriched >9xMagAttract DNA Blood Midi M48 Kit75 μl Buffy Coat50–75150–400
Buffy coat enriched ≤9xMagAttract DNA Blood Midi M48 Kit150 μl Buffy Coat100–150150–400
Buffy coat with low leukocyte
concentration
MagAttract DNA Blood Midi M48 Kit300 μl Buffy Coat200–300150–400

Procedure

MagAttract technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. Optimized buffers lyse samples, and DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt (see flowchart "MagAttract DNA Blood Mini M48 procedure"). DNA bound to the magnetic particles is then efficiently washed. Two different wash buffers are used, followed by a rapid rinse with distilled water, which considerably improves the purity of the DNA. High-quality DNA is eluted in the water provided.

Applications

DNA purified using the MagAttract DNA Blood Mini M48 Kit can be used in a wide range of downstream applications, including:

  • PCR and quantitative, real-time PCR
  • SNP genotyping
  • Sequencing

Specifications

Features
Specifications
Applications PCR, qPCR, real-time, genotyping, sequencing
Elution volume 50–400 µl
For automated processing BioRobot M48 Workstation
Format Sample tube (1.5 ml, 2 ml)
Main sample type Whole blood, buffy coat
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA
Sample amount 100–200 µl
Technology Magnetic particles
Time per run or per prep 23 minutes – 2.5 hours
Yield 3.9–6.2 µg

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