Innovative one-day workflow for simultaneous high-sensitivity profiling of DNA variants and RNA fusions


Part 1: Innovative one-day workflow for simultaneous high-sensitivity profiling of DNA variants and RNA fusions

Cancer is a highly complex disease and understanding the various alterations that together determine mechanisms of disease onset, progression, recurrence and response to treatment is challenging. Currently, multimodal approaches are used to explore biomarker status using DNA variant profiling and RNA fusion information. Though the information derived enables a better understanding of the various mechanisms in play, having to perform separate workflows is cumbersome and increases the overall cost of the study due to the number of instruments, reagents, consumables and resources needed. Furthermore, samples, especially those derived from tissues, are often of limited quantity and quality. Therefore, streamlined workflows for low sample inputs are preferred.

 

In this webinar, we explore a new workflow for simultaneous NGS profiling of both DNA variants and RNA fusions, as well as gene expression in just one day, using as little as 10 ng sample. We’ll discuss:

• Challenges of having two separate workflows on overall and hands-on time and cost

• How to maximize the information you derive from a single sample in half the time

• QIAseq Multimodal Panel overview and sample capabilities

• How our SPE-based technology ensures greater coverage, while Unique Molecular Index (UMI) technology enables high-sensitivity detection of DNA variants and RNA fusions, known and novel

• Performance data review