The DNeasy mericon 96 QIAcube HT Kit enables DNA extraction from food samples in radically shorter time compared to conventional CTAB protocols. Automated purification of 24–96 samples can be carried out on the QIAcube HT instrument in just a few hours with little hands-on time.
DNA was purified from peanut butter and chocolate using the DNeasy mericon Food Kit and kits from 3 other suppliers. [A] Quantitative PCR for the trnL chloroplast tRNA gene showed substantially lower average CT values for DNA purified with the DNeasy mericon Food Kit. [B] To test for carryover of inhibitors, DNA was diluted 1:10 and the same segment was amplified. DNA purified with the DNeasy mericon Food Kit was inhibitor-free, indicated by a ΔCT within the expected range of 2.9–3.5. DNA purified with the other kits gave much lower ΔCT values, indicating strong inhibitor contamination.
DNA was purified from orange juice, chocolate, and peanut butter using the QIAcube HT instrument and the DNeasy mericon 96 QIAcube HT Kit or manually using the DNeasy mericon Food Kit. Purified DNA was analyzed by qPCR of the trnL chloroplast tRNA gene using the QuantiFast SYBR® Green PCR Kit. The resulting CT values indicate similar performance from both purification methods. Diluting the input DNA 1:10 for qPCR analysis shows that all purified DNA was also free of PCR inhibitors.