DNeasy Plant procedure.
DNeasy Plant procedure.
Reproducible DNA yield and quality.
Reproducible DNA yield and quality.
Reliable PCR.
Reliable PCR.
DNA purity from pine needles.
DNA purity from pine needles.
DNeasy 96 Plant plate.
DNeasy 96 Plant plate.
Uniformity of DNA yields from 96 wheat samples.
Uniformity of DNA yields from 96 wheat samples.
DNeasy Plant procedure.
Reproducible DNA yield and quality. Genomic DNA was purified from different plant species following disruption in liquid nitrogen. DNA was eluted in 2 x 100 µl Buffer AE. Eluates (10 µl) were subjected to gel electrophoresis. Average yields are given under the plant names. M: lambda–HindIII.
Reliable PCR. Genomic DNA purified from different plant species was amplified by PCR. Eluate (5 µl) was used as template, and 30 µl of each PCR product were loaded per lane. M: markers.
DNA purity from pine needles. Spectrophotometric scans (220–320 nm) of DNA isolated from pine needles using the method of Dellaporta, CTAB, or the DNeasy Plant Mini Kit. Pure DNA typically shows a symmetrical peak at 260 nm and a smooth profile. Polysaccharides and other secondary metabolites, often copurified with plant DNA isolated using traditional methods, can interfere with OD readings (A260/A280), leading to errors in determination of concentration and purity.
DNeasy 96 Plant plate.
Uniformity of DNA yields from 96 wheat samples. Ninety-six leaf samples were collected from a batch of wheat plants grown under identical conditions. Samples (50 mg) were disrupted in liquid nitrogen and DNA was isolated using the DNeasy 96 Plant procedure.