Genomic DNA was purified from different plant species following disruption in liquid nitrogen. DNA was eluted in 2 x 100 µl Buffer AE. Eluates (10 µl) were subjected to gel electrophoresis. Average yields are given under the plant names. M: lambda–HindIII.
Genomic DNA purified from different plant species was amplified by PCR. Eluate (5 µl) was used as template, and 30 µl of each PCR product were loaded per lane. M: markers.
Spectrophotometric scans (220–320 nm) of DNA isolated from pine needles using the method of Dellaporta, CTAB, or the DNeasy Plant Mini Kit. Pure DNA typically shows a symmetrical peak at 260 nm and a smooth profile. Polysaccharides and other secondary metabolites, often copurified with plant DNA isolated using traditional methods, can interfere with OD readings (A260/A280), leading to errors in determination of concentration and purity.
Ninety-six leaf samples were collected from a batch of wheat plants grown under identical conditions. Samples (50 mg) were disrupted in liquid nitrogen and DNA was isolated using the DNeasy 96 Plant procedure.