Safety and handling considerations
Safety and handling considerations for animal cell culture
Legislation and regulatory guidelines
Safety considerations and biohazards
Handling cell cultures
Aseptic technique and minimization of aerosols
Laminar flow hoods
For the most efficient operation, laminar flow hoods should be located in an area of the laboratory where there is minimal disturbance to air currents. Avoid placing laminar flow hoods near doorways, air vents, or locations where there is high activity. Hoods are often placed in dedicated cell culture rooms.
- Keep laminar flow hoods clean, and avoid storing equipment inside the hood.
- Before starting work, disinfect the work surface of the hood as well as the outside of any bottles (e.g., by wiping with 70% ethanol), and then place everything needed for the cell culture procedure in the hood.
- Arrange equipment, pipets, waste containers, and reagent bottles so that used items are not placed near clean items, and avoid passing used items over clean items.
- Place used items (e.g., pipets) in a container inside the hood, and disinfect or seal before removing from the hood.