ipsogen JAK2 MutaScreen Kits

For qualitative or semi-quantitative detection of the JAK2 V617F/G1849T mutation using real-time PCR

Features

  • Reliable qualitative or semi-quantitative detection
  • Multiplex analysis with no need for optimization
  • Easy workflow with ready-to-use solutions

Useful Resources

Products

For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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ipsogen JAK2 MutaScreen RS Kit (19)

Cat. No. / ID: 673113

For 19 reactions: V617F Positive Control, V617F Negative Control, V617F Reference Scale, Primers and Probes Mix JAK2 wild-type and JAK2 V617F

Product Details

The ipsogen JAK2 MutaScreen Kits are intended for research use only and are not for use in diagnostic procedures. The kits are for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kits provide reagents optimized for reliable and sensitive detection, and qualitative or semi-quantitative analysis of the JAK2 V617F/G1849T mutation in genomic DNA.

Performance

To ensure highest accuracy, ipsogen JAK2 MutaScreen Kits have been optimized to detect the JAK2 V617F/G1849T mutation and use DNA positive and negative controls for qualitative analysis (see figures  Representative allelic discrimination experiments for qualitative detection of the JAK2 V617F/G1849T mutation in genomic DNA and  Representative allelic discrimination experiments for semi-quantitative detection of the JAK2 V617F/G1849T mutation in genomic DNA). ipsogen JAK2 MutaScreen Kits provide a DNA 2% cut-off sample (COS) positivity and a minimum of 25 ng of input sample DNA is recommended.
See figures

Principle

ipsogen JAK2 MutaScreen Kits are ready-to-use kits for the detection, qualitative, or semi-quantitative analysis of the JAK2 V617F/G1849T somatic mutation due to a single nucleotide polymorphism (SNP) in genomic DNA (see figure JAK2 mutation site identification). The kits provide reliable and reproducible results. ipsogen JAK2 MutaScreen Kits provide a DNA cut-off sample (COS), a DNA positive control (PC), and a negative control (NC) for JAK2 V617F. Use of the cut-off sample and controls enables accurate qualitative analysis of genotyping results. The ipsogen JAK2 MutaScreen RS Kit provides a V617F reference scale.

Procedure

The first step is extraction and determination of quality and quantity of genomic DNA. The second step is the amplification of DNA by real-time PCR. Using the ipsogen JAK2 MutaScreen Kits provide reliable detection, qualitative, or semi-quantitative analysis of the JAK2 V617F/G1849T mutation. Simply start the reaction using the optimized protocols described in the kit handbook.

Applications

ipsogen JAK2 MutaScreen Kits enable sensitive and reliable detection, qualitative, or semi-quantitative analysis of the JAK2 V617F/G1849T mutation for research use only.

Supporting data and figures

Resources

Kit Handbooks (1)