text.skipToContent text.skipToNavigation

ipsogen BCR-ABL1 mbcr Kit CE

For quantitative detection of BCR-ABL mbcr e1a2 transcripts using real-time PCR
  • Highly accurate and sensitive quantitative assay
  • EAC standardized assay for reproducible results
  • Compliance with EU IVD Directive 98/79/EC
  • Easy workflow with ready-to-use solutions
The ipsogen BCR-ABL1 mbcr Kit is an in vitro molecular diagnostic kit for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kit provides reagents optimized for reliable and sensitive detection and quantification of BCR-ABL mbcr p190 e1a2 transcripts in bone marrow or peripheral blood samples of Ph-positive acute lymphoblastic leukemia (ALL) patients previously diagnosed with a BCR-ABL mbcr fusion gene event. The test is intended to monitor efficacy of treatment in patients undergoing therapy and for minimal residual disease (MRD) follow-up to monitor disease relapse.
Cat No./ID: 670023
ipsogen BCR-ABL1 mbcr Kit (24) CE
For 24 reactions: ABL Control Gene Standards, BCR-ABL mbcr Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix BCR-ABL mbcr Fusion Gene
Performance
To ensure highest sensitivity, the ipsogen BCR-ABL1 mbcr Kit has been optimized to detect BCR-ABL mbcr p190 e1a2 and ABL transcripts and uses prediluted plasmid standards and primers and probe mixes (see figures Accurate detection of BCR-ABL mbcr plasmid standards and Reliable detection of ABL plasmid standards).
Principle
The ipsogen BCR-ABL1 mbcr Kit is a ready-to-use kit for the detection of BCR-ABL mbcr p190 e1a2 fusion gene transcripts using real-time PCR. The kit is based on the amplification and detection of specific BCR-ABL p190 e1a2 transcripts, relative to ABL control gene expression, in total RNA extracted from bone marrow or peripheral blood samples of Ph-positive acute lymphoblastic leukemia (ALL) patients previously diagnosed with a BCR-ABL mbcr fusion gene event (see figures BCR-ABL mbcr fusion gene transcript and ABL control gene transcript). The kit provides high levels of specificity, sensitivity, and reproducibility. The ipsogen BCR-ABL1 mbcr Kit provides 5 standard dilutions for BCR-ABL mbcr and 3 standard dilutions for ABL. Use of the standards enables accurate quantification of transcripts. The technology used for quantification of BCR-ABL mbcr has been standardized according to Europe Against Cancer (EAC) recommendations and the ipsogen BCR-ABL1 mbcr Kit uses this validated technology to calibrate results.
Procedure
The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using the ipsogen BCR-ABL1 mbcr Kit allows detection and quantification of BCR-ABL mbcr p190 e1a2 and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.
Applications
The ipsogen BCR-ABL1 mbcr Kit enable sensitive and reliable detection and quantification of BCR-ABL mbcr p190 e1a2 transcripts, relative to ABL control gene expression, for in vitro diagnostic use.

You are not authorized to download the resource

Operating Software (2)
Gamma Plug-in for use with Rotor-Gene AssayManager v2.1
Show details
To download Rotor-Gene AssayManager v2.1 after purchase:
    Click the "Download" button and follow the on-screen instructions to install Rotor-Gene AssayManager v2.1.
    Rotor-Gene AssayManager v2.1 and license must be purchased prior to use.    

    IMPORTANT: In order to perform and analyze runs, at least one Rotor-Gene AssayManager v2.1 plug-in needs to be installed. Refer to your assay kit handbook for more information.

To purchase a license key for Rotor-Gene AssayManager v2.1:
    1. After purchasing Rotor-Gene AssayManager v2.1 software and licenses, click the "Download" button and follow the onscreen instructions to install. After successful installation of the Rotor-Gene AssayManager v2.1 and a plug-in, a “key file” will be generated. This "key file" will be attached to the "Technical Support Form" in Step 5, below.   
    2. Go to the Technical Support Form. Make sure to include all required information and your Rotor-Gene Q or Rotor-Gene Q MDx serial number when filling out the form.       
    3. For Question 2, "Type of Request", select “Other”.
    4. For Question 4, “Your inquiry”, enter "Rotor-Gene AssayManager license request” and include your purchase order number, from your order confirmation.
    5. Include the "key file" from Step 1 as an attachment.
    6. Submit the form. QIAGEN Technical Services will respond via email with a license file within 1–2 working days.    
    7. After receiving your computer-specific Rotor-Gene AssayManager v2.1 license file, archive the license file locally on your computer.

IMPORTANT: The license file must always exist on the computer that has Rotor-Gene AssayManager v2.1 installed. The license file location should be protected and not subject to change.


Show details
fragment fix placeholder