Expanding the Dynamic Range of microRNA Expression: Quantification from Liquid Biopsy

Improved nucleic acid quantitation from precious samples is a key driver for the concept of liquid biopsy. By quantifying miRNA from serum/plasma samples as well as biofluids such as cerebral spinal fluid, key biomarkers can be detected without the need for invasive tissue-based biopsy. In this online seminar recording of the Sept. 10 webinar, Jo Vandesompele of the Center for Medical Genetics at Ghent University provides an overview of the use of preamplification and minimum sample inputs to maximize the dynamic range of miRNA expression studies. Jon Shaffer of QIAGEN follows with a discussion of microRNA detection technology and platform comparison. Watch it on-demand!

Jo Vandesompele

Jo Vandesompele has a Master of Science in Bio-engineering (1997, UGent), a Ph.D. in Medical Genetics (2002, UGent), and has been a professor in functional genomics and applied bioinformatics at Ghent University since 2007. He is author of more than 140 scientific articles in international journals, including some pioneering publications in the domain of real-time PCR. Together with Jan Hellemans he developed advanced and universally applicable quantification methods for state-of-the-art qPCR data analysis. He is also one of the founders of the RDML consortium and co-author of the MIQE guidelines.
Jonathan Shaffer, Ph.D.

Jonathan M. Shaffer
Dr. Shaffer joined QIAGEN in 2009 and has since worked with various technology development groups, the most recent being miRNA technologies. He received his Ph.D. in biochemistry and molecular genetics from the University of Pittsburgh School of Medicine in 2008 where his research focused on determining the mechanisms that regulate non-receptor tyrosine kinase expression and activity. Dr. Shaffer did his postdoctoral training at SABiosciences Corporation, now part of QIAGEN. Currently, Dr. Shaffer is a Senior Scientist in Product Development at QIAGEN.