Single Base Resolution DNA Methylation and Mutation Analysis in Long Sequence Runs

Pyrosequencing is a highly flexible technology that lets you rapidly analyze short- to medium-length sequences fast and quantitatively with high accuracy. The real-time, high-resolution sequence output makes the technology highly suitable for applications including complex mutation analysis, microbial identification, and DNA methylation quantification.

The main bottleneck in Pyrosequencing has been limited sequence length, which is critical for some applications. Our new technology, software, and chemistry overcome this bottleneck and give sequence reads that are typically twice as long as those from previous PyroMark systems. The new PyroMark Q24 Advanced system also reduces background noise, improving quantification even at sites distant from the sequencing start. The new system is ideal for applications requiring analysis of longer sequences, such as DNA methylation analysis in epigenetic research, frequency determination in mutation analysis, and various de novo sequencing applications.

In this presentation, we will discuss the following applications and technology improvements:
  • DNA methylation analysis at single base resolution at CpG and CpN sites
  • Improved quantification of sequence variations at any sequence position
  • Easy and improved base calling functionality

Dr. Gerald Schock, Associate Director, Digital PCR Instruments, QIAGEN GmbH

Gerald Schock

Gerald Schock studied Biology and Biochemistry at the University of Mainz, Germany and at the University College of Wales, Aberystwyth, UK. He joined QIAGEN after receiving his Ph.D. in 1996, and has held positions in Sales, Regional Marketing, and Global Marketing, where he built up the epigenetics portfolio including Pyrosequencing instruments before assuming responsibility for the Life Science detection portfolio, and has now transitioned into digital PCR.