Plasmid DNA was used as a template for PCRs run on the Rotor-Gene Q. CT values of reactions in open tubes without caps overlaid with Vapor-Lock are shown in red. CT values of reactions in capped tubes are shown in blue. Reactions containing plasmid DNA as template are indicated with squares and no-template controls are indicated with triangles. Four replicate reactions were performed and standard deviations are shown. Reactions in open tubes and capped tubes performed similarly (mean CT values of 16.29 and 16.85 respectively). No-template control reactions also resulted in similar CT values for open and capped tubes (mean CT values of 32.30 and 31.32 respectively).
Fluorescence was measured on the green channel during a run with the Rotor-Gene Q. Fluorescence was measured from tubes containing Vapor-Lock (red), tubes containing water (blue), and empty tubes (green). Tubes containing Vapor-Lock provided similar results to the other tubes tested. Similar results were obtained for all five other channels on the Rotor-Gene Q (data not shown).
Human genomic DNA was used as template for amplification of the human bcl-2 oncogene. The total reaction volume was 5 µl. Reactions were performed in uncapped 0.1 ml Strip Tubes with (red) and without (blue) a 10 µl overlay of Vapor-Lock. Five replicate reactions were performed. Data was acquired on the green channel. Vapor-Lock overlay was essential for amplification success of the reactions. Only samples using the Vapor-Lock overlay resulted in detectable amplification (mean CT = 26.23, SD = 0.10).