Investigator Quantiplex Kits

For rapid and reliable detection, quantification and qualitative assessment of human and male DNA with high sensitivity

Features

  • Simultaneous quantification of total human or human and male DNA 
  • Reliable results with sensitivity down to <1 pg/µl DNA 
  • High sensitivity for male DNA in a female background 
  • Quantiplex Pro Kits: Straightforward quality assessment for reliable information on DNA degradation, which correlates with your STR profiles 
  • Integrated internal control to test for PCR amplification or inhibition 
Investigator Quantiplex Kit (200)

Cat. No. / ID: 387016

Primer Mix IC FQ, Reaction Mix FQ, Control DNA Z1, Dilution Buffer
€581.00
Kit
Quantiplex
Quantiplex Pro
Quantiplex Pro Calibration
Quantiplex Pro RGQ
Quantiplex HYres
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Investigator Quantiplex Kits are intended for molecular biology applications in forensic, human identity and paternity testing. These products are not intended for diagnosis, prevention or treatment of a disease.

Product Details

Investigator Quantiplex Kits are ready-to-use quantitative real-time PCR assays for human identity testing and forensic applications. Developed for superior sensitivity and reaction speed, they are designed to establish whether a sample contains sufficient and intact DNA to enable DNA fingerprinting analysis (such as STR, SNP or DIP analysis) and whether there are inhibitors present. Kits are available for quantification of human DNA, or quantification of total human and male DNA. The Investigator Quantiplex Pro technology further enables quality assessment to provide reliable information on the degradation status of your sample. The new IC (Internal Control) in Investigator Quantiplex Pro Kits is more sensitive toward inhibitors and correlates to the STR tolerance while the quantification result remains reliable.

Investigator Quantiplex Kits meet ISO 18385 requirements.

Want to try the Investigator Quantiplex Pro Kit for the first time? Request a quote for a trial kit.

Want to try the Investigator Quantiplex Pro RGQ Kit for the first time? Request a quote for a trial kit.

Performance

Investigator Quantiplex Pro Kits provide robust and precise quantification results with high sensitivity within a high dynamic range. In addition to the general sensitivity, the kits provide exceptionally high sensitivity for male DNA, even in a very high background of female DNA (up to 400,000:1).

The straightforward quality assessment system included in the kits comprises a newly developed DNA Degradation Control (DC) and a newly designed Internal Control (IC). The new DNA Degradation Control provides superior detection of DNA degradation (see figures “ Accurate detection of DNA degradation” and “ Accurate detection of male DNA degradation in a sample mixture”). Furthermore, the Investigator Quantiplex Pro RGQ assay is the only commercially available assay that can distinguish between human and male DNA degradation (see figure “ Highly accurate and sensitive detection results for male and total human DNA”). The newly designed Internal Control reliably detects potential PCR inhibitors. The IC is more sensitive to inhibitors and correlates to STR tolerance while the quantification results remain reliable (see figure “ Identification of PCR inhibition”). Furthermore the IC of the Investigator Quantiplex Pro Kits is analogous to the Quality Sensor of Investigator STR QS PCR Assays (e.g., Investigator 24plex QS Kits, Investigator 26plex QS Kits, Investigator ESSplex SE QS Kit and Investigator Argus X-12 QS Kit).

See figures

Principle

Human identification is commonly based on the analysis of short tandem repeats (STRs), single nucleotide polymorphisms (SNPs) or deletion insertion polymorphisms (DIPs), depending on the demands of an examination or on the sample quality. These multiplex assays are complex systems that require a defined range of template input.

Investigator Quantiplex Pro Kits are a ready-to-use system for the detection of human and male DNA and parallel assessment of DNA degradation using quantitative real-time PCR. The kits provide fast and accurate quantification of human DNA from forensic database and casework samples. The kits contain reagents and a DNA polymerase for specific amplification of 4NS1C, a 91 bp proprietary region present on several autosomes of the human genome, and for detection of the specific PCR products on Applied Biosystems 7500 Real-Time PCR Systems or the Rotor-Gene Q System.  

Information on the targets, amplicon lengths, channels, ploidy and copy numbers is summarized in the table “Targets, amplicon lengths and channels for Investigator Quantiplex Kits”. 

Targets, amplicon lengths and channels for Investigator Quantiplex Kits

Kit Target Amplicon length Channel Ploidy Copy number
Quantiplex Human target 146 bp FAM Diploid Multi-copy
Internal PCR control 200 bp VIC N.A. Synthetic fragment
Quantiplex HYres Human target 146 bp FAM Diploid Multi-copy
Human male target 129 bp Cy5 Haploid Multi-copy
Internal PCR control 200 bp VIC N.A. Synthetic fragment
Quantiplex Pro Human target, small autos. 91 bp FAM Diploid Multi-copy
Human target, large autos. 353 bp TAMRA/Atto550 Diploid Multi-copy
Human male target 81 bp CY5/Atto647N Diploid Multi-copy
Internal PCR control 434 bp JOE N.A. Synthetic fragment
Quantiplex Pro RGQ Human target, small autos. 91 bp Yellow Diploid Multi-copy
Human target, large autos. 353 bp Red Diploid Multi-copy
Human male, small Y 81 bp Green Haploid Multi-copy
Human male, large Y 359 bp Orange Haploid Multi-copy
Internal PCR control 434 bp Crimson N.A. Synthetic fragment

Procedure

The kits are a ready-to-use system for the detection and quantification of human DNA using real-time PCR. See the table "Technical specifications" for more information. A set of template files has been developed to streamline the instrument setup and the analysis of the results of Investigator Quantiplex Kits on the Rotor-Gene Q (see table "Template files available for the Rotor-Gene Q").

Applications

Investigator Quantiplex Kits are for quantifying human and male DNA, and for determining if PCR inhibitors or DNA degradation are present and if further sample purification is needed. They are suitable for:

  • Forensic database and casework samples
  • Paternity testing samples
  • Sexual assault samples
  • Disaster victim identification and anthropological work
  • Other human identity testing applications

Technical specifications

All targets are multi-copy targets.

Features Investigator Quantiplex Pro Kit Investigator Quantiplex Pro RGQ Kit Investigator Quantiplex HYres Kit Investigator Quantiplex Kit
Volume per PCR 20 µl 20 µl 20 µl 25 µl
Input volume of template DNA 2 µl 2 µl 2 µl 2 µl
Human target, small autosomal 91 bp 91 bp 146 bp 146 bp
Human target, large autosomal 353 bp 353 bp - -
Human male target, small Y 81 bp 81 bp 129 bp -
Human male target, large Y - 359 bp - -
Probes TaqMan TaqMan Scorpions Scorpions
Real-time cyclers Applied Biosystems 7500 Real-Time System or Applied Biosystems 7500 Fast Real-Time System

Rotor-Gene Q, 5plex or 6plex only

 

Rotor-Gene Q, 5plex or 6plex only, Applied Biosystems 7500 Real-Time System, or Applied Biosystems 7500 Fast Real-Time System

Rotor-Gene Q, Applied Biosystems 7500 Real-Time System, or Applied Biosystems 7500 Fast Real-Time System 

Template files available for the Rotor-Gene Q

Template file Purpose
Investigator Quantiplex Pro Template Files ABI 7500 instrument setup and the analysis of the results
Investigator Quantiplex Pro RGQ Q-Rex Templates instrument setup and the analysis of the results
Investigator Quantiplex HYres Kit Cycling Preset cycling protocol
Investigator Quantiplex HYres Kit Sample Sample name, sample type and concentration for standard curve and internal control
Investigator Quantiplex Kit HYres Analysis Settings for the Yellow Channel CT settings
Investigator Quantiplex Kit Cycling Preset cycling protocol
Investigator Quantiplex Kit Sample Sample name, sample type and concentration for standard curve and internal control
Investigator Quantiplex Kit Analysis Settings for the Yellow Channel CT settings

Supporting data and figures

Resources

Scientific Posters (5)
Brochures & Guides (2)
Explore our new, easy-to-navigate digital version of the Investigator Quantiplex Pro Kit Product Profile!
Kit Handbooks (4)
For quantification of human and male DNA in forensic samples
For quantification of human DNA in forensic samples
For quantification of human and male DNA in forensic samples

FAQ

Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?
Versions starting from SDS v. 1.2.3 can be used to run the Investigator Quantiplex. However, if using software version 1.2.3, be sure to uncheck the box “9600 Emulation” in the cycling set-up.
FAQ ID -2569
How long can I store the dilutions of the Control DNA Z1 standards at 2°C?
If the series of Control DNA Z1 standards was diluted using the provided QuantiTect Nucleic Acid Dilution Buffer, then they can be stored for at least one week at 2-8°C.
-8
Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?
No, you do not. Please use the FAM and the VIC dyes as explained in the Investigator QuantiPlex Kit handbook.
FAQ ID -2571
Which human target is used for the quantification in the Investigator Quantiplex Kit?
The 4NS1C target is a multi-copy target which was validated in an external study. It is represented by about 20 copies per haploid genome and is located on different autosomal chromosomes.
FAQ ID -2577
How long are the target sequences of the Investigator Quantiplex Pro RGQ Kit?
The amplified regions on the human genome are 91 bp and 353 bp, the male specific targets are 81 bp and 359 bp, and the Internal Control is 434 bp.
FAQ ID - 3746
On which real-time cyclers is the Investigator Quantiplex Pro Kit validated?
The Investigator Quantiplex Pro Kit is validated on the Applied Biosystems 7500 Standard and 7500 Real-Time HID PCR Systems.

FAQ ID - 3728
What do I have to consider if I am using Applied Biosystems SDS software version 1.2.3 to run an Investigator Quantiplex reaction?
If using software version 1.2.3, be sure to uncheck the box “9600 Emulation” in the cycling set-up.
FAQ ID -2570
What is the detection limit for the Investigator Quantiplex Kit?
The detection limit for the Investigator Quantiplex Kit is a DNA concentration in the sample of 0.3 pg/µl.
FAQ ID -2572
How long are the target sequences of Investigator Quantiplex Pro?
The amplified regions on the human genome are 91 bp and 353 bp; the male specific target is 81 bp and the internal control is 434 bp.
FAQ ID - 3735
At what temperature should I store the Investigator Quantiplex Kit?
The QuantiTect Dilution Buffer should be stored at –20°C. All of the other components can be stored at 2-8°C.
FAQ ID -2578
How long can I store the dilutions of the Male Control DNA M1 standards at 2–8°C?
If the series of the Male Control DNA M1 standards was diluted using the QuantiTect Nucleic Acid Dilution Buffer provided, then they can be stored for at least one week at 2–8°C. 
FAQ ID - 3744
What do I have to consider if I am using the Applied Biosystems SDS software version 1.2.3 in order to run an Investigator Quantiplex Pro reaction?
If using software version 1.2.3, be sure to uncheck the box “9600 Emulation” in the cycling setup.
FAQ ID - 3731
Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?
The Internal Control is designed to report if inhibitors are contained in the DNA sample. A shift in the CT value in comparison to the dilution series of the Male Control DNA M1 is an indication of potential inhibition in the DNA sample.
FAQ ID - 3745
Which real-time cycler software version can be used to run the Investigator Quantiplex Pro Kit?

Using the Investigator Quantiplex Pro Kit on the Applied Biosystems 7500 Real-Time PCR System:

All software versions starting with SDS v. 1.2.3 can be used. If using software version 1.2.3, be sure to uncheck the box “9600 Emulation” in the cycling setup.
FAQ ID - 3730
Which real-time cycler software version can be used to run the Investigator Quantiplex Pro RGQ Kit?

Using the Investigator Quantiplex Pro RGQ Kit on the Rotor-Gene Q System:

all software versions starting from Q-Rex 1.0 can be used.
FAQ ID - 3743
Why do I observe a CT shift for the Internal Control in some samples in comparison to the Ct value for the Internal Control of the dilution series of the Control DNA Z1?
The Internal Control is designed to report if inhibitors are contained in the DNA sample. A shift in the Ct value in comparison to the dilution series of the Control DNA Z1 is an indication of potential inhibition in the DNA sample.
FAQ ID -2575
How long are the target sequences of Investigator Quantiplex?
The amplified region on the human genome is 146 bp and the Internal Control is 200 bp.
FAQ ID -2576
Which Rotor-Gene Q System can be used to run the Investigator Quantiplex Pro RGQ Kit?
The Investigator Quantiplex Pro RGQ Kit can only be run on the Rotor-Gene Q System (5plex/6plex) with 72-Well Rotor/Rotor-Disc 72/Rotor-Disc 72 Rotor and Rotor-Disc 100/Rotor-Disc 100 Rotor.
FAQ ID - 3742
Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
Be sure to perform fluorescence data collection using the green and the yellow channels with auto-gain optimization.
FAQ ID -2574
Do I have to calibrate new dyes on my Applied Biosystems 7500 or 7500 Fast Real-Time PCR System?
With the Investigator Quantiplex Pro Kit, custom dye calibration for FAM, JOE, ATTO 550, ROX, and ATTO 647N is strongly recommended when using the Applied Biosystems 7500 Real-Time PCR System. To achieve optimal performance, please calibrate with the Investigator Quantiplex Pro Calibration Kit (cat. no. 387416). However, standard dye calibrations for FAM, JOE, TAMRA, ROX, and Cy5 might be used. In this case, use TAMRA for the Degradation target and Cy5 for the Male target.
FAQ ID - 3732
How long does an Investigator Quantiplex run take?
On the Rotor-Gene Q, an Investigator Quantiplex run of 40 cycles takes approximately 48 minutes. On the Applied Biosystems 7500 Real-Time PCR System, an Investigator Quantiplex run of 40 cycles takes approximately 57 minutes
FAQ ID -2566
How long can I store the dilutions of the Male Control DNA M1 standards at 8°C?
If the series of the Male Control DNA M1 standards was diluted using the QuantiTect Nucleic Acid Dilution Buffer provided, then they can be stored for at least one week at 2–8°C.
FAQ ID - 3733
For which real-time cyclers has the Investigator Quantiplex Kit been validated?
The Investigator Quantiplex Kit has been validated for the Rotor-Gene Q, and the Applied Biosystems 7500 Fast and 7500 Real-Time PCR Systems.
FAQ ID -2567
At what temperature should I store the Investigator Quantiplex Pro RGQ Kit?
The Investigator Quantiplex Pro RGQ Kit is stored at –15°C to –30°C. After first time use, store kit components at 2–8°C. Avoid freezing the kit components. The QuantiTect Nucleic Acid Dilution Buffer may also be stored at –15°C to –30°C, if desired.
FAQ ID - 3748
Which human target is used for the quantification?
The 4NS1C target is a multi-copy target, which was validated in an external study. It is located on different autosomal chromosomes.
FAQ ID - 3736
Which version of the Rotor-Gene Q software can be used to run the Investigator Quantiplex?
Using the Investigator Quantiplex on the Rotor-Gene Q: All versions of the Rotor-Gene Q software can be used to run the Investigator Quantiplex.
FAQ ID -2568
How long does an Investigator Quantiplex Pro run take?

Using the Investigator Quantiplex Pro Kit on the Applied Biosystems 7500 Real-Time PCR System.

40 cycles, approximately 60 minutes
FAQ ID - 3727
How can I streamline the quantification workflow?
You can download the template files for Investigator Quantiplex Pro from the website and use them according to the handbook. These files will allow you to use the right parameters to run a quantification reaction and analyze the results in the proper way.
FAQ ID - 3738
Can the Investigator Quantiplex Pro Kit be run on on the Rotor-Gene Q?
No, the dyes are not compatible with the Rotor-Gene Q. A separate Rotor-Gene Q version is available:
Investigator Quantiplex Pro RGQ Kit (200) (Cat. No./ID: 387316)
FAQ ID - 3729
At what temperature should I store the Investigator Quantiplex Pro Kit?
The Investigator Quantiplex Pro Kit is stored at –15°C to –30°C. After first time use, store kit components at 2–8°C. Avoid freezing these kit components. The QuantiTect Nucleic Acid Dilution Buffer may also be stored at –15°C to –30°C, if desired.
FAQ ID - 3737
Which human target is used for the quantification?
The 4NS1C target is a multi-copy target, which was validated in an external study. It is located on different autosomal chromosomes.
FAQ ID - 3747
Why do I observe a CT shift for the internal control in some samples in comparison to the CT value for the internal control of the dilution series of the Male Control DNA M1?
The internal control is designed to report if inhibitors are contained in the DNA sample. A shift in the CT value in comparison to the dilution series of the Male Control DNA M1 is an indication of potential inhibition in the DNA sample.
FAQ ID - 3734
On which real-time cyclers is the Investigator Quantiplex PRO RGQ Kit validated?
The Investigator Quantiplex Pro RGQ Kit is validated on the Rotor-Gene Q System.
FAQ ID - 3741
How long does an Investigator Quantiplex Pro RGQ run take?

Using the Investigator Quantiplex Pro RGQ Kit on the Rotor-Gene Q System – 40 cycles, about 1 hour.

FAQ ID - 3740
How can I streamline the quantification workflow for the Investigator QuantiPlex Kit?
You can download the template files for the Investigator Quantiplex Kit from the website [add link] and use them according to the handbook. These files provide the correct/optimal parameters to run a quantification reaction and enable proper result analysis.
FAQ ID -2579
How can I streamline the quantification workflow?
You can download the template files for the Investigator Quantiplex Pro RGQ Kit from the website and use them according to the handbook. These files will allow you to use the right parameters to run a quantification reaction and analyze the results in a proper way.

FAQ ID - 3749