QIAsymphony mericon Bacteria Kit

For automated isolation of pathogen DNA from primary bacterial enrichment cultures

Features

  • Suitable for bacterial DNA isolation from all relevant food and environmental samples
  • DNA isolation from Gram-negative or Gram-positive bacteria with one protocol
  • No inhibitor carryover, so pathogen DNA is ready for use in downstream applications
  • Increases productivity by minimizing hands-on time
  • Easy tracking of samples and reagents using bar codes
QIAsymphony mericon Bacteria Kit (360)

Cat. No. / ID: 931156

For 360 preparations: 2 Reagent Cartridges, Piercing Lid, TopElute Fluid (60 ml), Reuse Seal Set
€909.00
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QIAsymphony mericon Bacteria Kit are intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The QIAsymphony mericon Bacteria Kit is for automated isolation of bacterial DNA from primary enrichment cultures of food and environmental samples as part of a pathogen screening workflow. It is specifically designed for use on the QIAsymphony SP, and optimized for use with the mericon Pathogen Detection real-time PCR assays. It uses a single protocol that is suitable for DNA isolation from Gram-negative or Gram-positive bacteria and features high process safety, including an inhibitor removal step and bar code reading to enable full tracking of reagents and samples. Each kit has reagents and accessories for 360 reactions. Prefilled reagent cartridges and continuous sample loading reduce setup time and minimize manual handling.

Performance

The QIAsymphony mericon Bacteria Kit is specifically designed for use on the QIAsymphony SP and optimized for use with the mericon Pathogen Detection real-time PCR assays. Up to 96 samples can be loaded onto the QIAsymphony SP at a time. The samples are processed in batches of 24 with a 53-minute processing time per batch.

Each kit contains reagents and accessories for 360 reactions. The reagents are provided in resealable reagent cartridges for maximum flexibility.

Principle

The kit was specifically designed for use on the QIAsymphony SP, which is a sample preparation instrument that is part of the modular QIAsymphony RGQ system. The automated protocol allows the isolation of bacterial DNA from primary enrichment cultures of food, animal feed, and environmental samples. Bacteria concentrated by filtration can also be used as a starting material. The protocol is based on proven magnetic-particle technology that allows the isolation of DNA from both Gram-negative and Gram-positive bacteria.

The DNA extraction technology of the QIAsymphony SP instrument combines the speed and efficiency of silica-based nucleic acid purification with the convenient handling of magnetic particles. The protocol involves a short thermal lysis procedure that includes an inhibitor removal step. This is followed by a highly effective and streamlined process of binding, washing, and elution, using silica-based magnetic particles in combination with optimized buffers. The isolated DNA is ready for use in downstream applications, such as real-time PCR-based pathogen detection.

A complete, automated workflow is possible on the QIAsymphony RGQ, as the eluates containing the purified pathogen DNA can be automatically transferred to the assay setup module (QIAsymphony AS). These assay plates can then be transferred to the Rotor-Gene Q thermal cycler for real-time PCR and data analysis.

Procedure

No pretreatment of the bacterial enrichment culture or filtration-concentrated bacteria is needed. Samples are extracted directly from the culture and loaded onto the QIAsymphony SP along with the reagents and plasticware. The instrument performs all of the steps of the pathogen DNA isolation procedure and can process up to 96 samples in a single run. The protocol comprises 4 steps: lysis, binding, washing, and elution. Worktable setup is rapid and the instrument allows continuous sample loading, which minimizes hands-on time for increased productivity.

Applications

The QIAsymphony mericon Bacteria Kit is for automated pathogen DNA isolation from primary enrichment cultures of food and environmental samples as part of a real-time PCR-based pathogen detection workflow.

FAQ

What samples can the QIAsymphony mericon Bacteria Kit be used for?
The QIAsymphony mericon Bacteria Kit can be used to isolate DNA from primary enrichment cultures of Gram-negative and Gram-positive bacteria and from bacteria concentrated by filtration.
FAQ ID -2958
Is RNA co-purified using the QIAsymphony mericon Bacteria Kit?
In principle, RNA co-purification is possible using the QIAsymphony mericon Bacteria Kit. However, at the beginning of the protocol, the reaction solution is heated to 90°C for 5 min to lyse the bacteria. If it is not completely destroyed, any RNA should be strongly depleted.
FAQ ID -2960
What happens if the starting volume is too low when using QIAsymphony mericon Bacteria Kit?
If the volume of the starting material is below 400 µl, the QIAsymphony mericon Bacteria Kit cannot process the sample, and the corresponding well of the elution plate will remain empty.
FAQ ID -2962
Do you need to pre-treat food samples before DNA isolation using the QIAsymphony mericon Bacteria Kit on the QIAsymphony SP?
No pretreatment is necessary using the QIAsymphony mericon Bacteria Kit on the QIAsymphony SP?
FAQ ID -2957
Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
Is food DNA co-purified using the QIAsymphony mericon Bacteria Kit?
Processed foods always contain high concentrations of DNA that has been liberated during industrial processing. In principle, this is co-purified during the QIAsymphony mericon Bacteria Kit protocol. However, in a sufficiently grown enrichment culture, the amount of free food DNA is negligible compared to the concentration of enriched bacteria.
FAQ ID -2959
How many reactions can be performed with one QIAsymphony mericon Bacteria Kit?
Each QIAsymphony mericon Bacteria Kit contains reagents for 360 reactions.
FAQ ID -2955
What happens if the starting volume is too high when using QIAsymphony mericon Bacteria Kit? Can spillage occur when the sample is initially transferred?
The QIAsymphony performs a liquid level detection before the sample is transferred. The pipet enters the tube to a depth of approximately 2 mm and measures the overall volume. Therefore, sample spillage when using QIAsymphony mericon Bacteria Kit is highly unlikely although not impossible.
FAQ ID -2963
What is the starting volume for the QIAsymphony mericon Bacteria Kit?
The starting volume for DNA isolation with the QIAsymphony mericon Bacteria Kit is 400 µl of the bacterial enrichment culture.
FAQ ID -2956
Does the QIAsymphony mericon Bacteria Kit contain an extraction control?
No, there is no extraction control included in the QIAsymphony mericon Bacteria Kit.
FAQ ID -2961