The Pyrogram shows the mutation (nucleotide 34 indicated with an arrow) with the "Sequence to Analyze" GNTGNTGTTGGGAAAAGC targeting base 2 in codon 12 (nucleotide 35). The red box indicates that this sequence is unexpected and needs to be checked.
The mutation GGT to AGT was reanalyzed with the "Sequence to Analyze" NGTNGTGTTGGGAAAAGC targeting base 1 in codon 12 (nucleotide 34).
Linearity of mutation GGT->GAT in codon 12.
The sequence indicated is the analyzed sequence for a normal sample. FP: Forward PCR primers; RPB: Reverse PCR primers (B indicates biotinylation); Seq: Sequencing primers.