A time-saving, streamlined protocol delivers RNA libraries – ready for use on Illumina NGS platforms.
A time-saving, streamlined protocol delivers RNA libraries – ready for use on Illumina NGS platforms.
Single cell RNA sequencing reveals cellular heterogeneity.
Single cell RNA sequencing reveals cellular heterogeneity.
Superior RNA-Seq library quality and reproducibility with a high percentage of protein-coding reads.
Superior RNA-Seq library quality and reproducibility with a high percentage of protein-coding reads.
High number of genes is detected, independent of input cell number.
High number of genes is detected, independent of input cell number.
A time-saving, streamlined protocol delivers RNA libraries – ready for use on Illumina NGS platforms. The REPLI-g Single Cell RNA Library Kit provides a complete WTA workflow – from cell lysis, gDNA removal and cDNA synthesis to highly uniform amplification across the entire transcriptome in a one-tube protocol – with negligible sequence bias. This is followed by fast, one-tube library construction without cleanup steps between different enzymatic reactions.
Single cell RNA sequencing reveals cellular heterogeneity. Sequencing libraries were generated from 3 individual HeLa S3 cells using the REPLI-g Single Cell RNA Library Kit and sequenced on an Illumina sequencer. The plot shows the number of genes detected in each single cell.
Superior RNA-Seq library quality and reproducibility with a high percentage of protein-coding reads. RNA-Seq libraries were prepared in triplicate from 1 or 10 HeLa S3 cells and in duplicate from 25 HeLa S3 cells using the REPLI-g Single Cell RNA Library Kit, and sequenced on an Illumina platform. The percentage of RNA biotypes detected in each library is plotted.
High number of genes is detected, independent of input cell number. RNA-Seq libraries were prepared from 1, 3, 10, 25 or 50 HeLa S3 cells using the REPLI-g Single Cell RNA Library Kit and sequenced on an Illumina platform.