QIAamp MinElute Virus Spin Kit
For simultaneous purification of viral DNA and RNA from plasma, serum, and cell-free body fluids
The QIAamp MinElute Virus Spin Kit simplifies purification of viral DNA and RNA with fast spin-column procedures. The QIAamp MinElute Virus Spin Kit uses starting sample volumes of up to 0.2 ml and combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 μl. The QIAamp MinElute Virus Spin process can be fully automated on the QIAcube Connect. Buy Products
The QIAamp MinElute Virus Spin Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Purified viral nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications such as PCR and RT-PCR (see figures "High sensitivity in PCR" and "High sensitivity in RT-PCR"). Viral nucleic acids purified using the QIAamp MinElute Virus Spin Kit can be used in a wide range of downstream applications, including:
Principle
The QIAamp MinElute Virus Spin Kit simplifies isolation of viral RNA and DNA from plasma, serum, and cell-free body fluids with a fast spin-column procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp MinElute technology yields viral RNA and DNA from plasma, serum, and cell-free body fluids ready to use in PCR and blotting procedures. The QIAamp MinElute Spin Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. The procedure is suitable for use with plasma, serum, and other cell-free body fluids. QIAamp sample preparation technology is fully licensed.
Procedure
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp MinElute membrane (see flowchart "QIAamp MinElute Virus Spin procedure"). Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and viral nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases, and other impurities.
Applications
The QIAamp MinElute Virus Spin Kit uses well-established technology for simultaneous purification of viral RNA and DNA from:
Specifications
Product Resources |
