QIAseq Targeted RNA Extended Panels
QIAseq Targeted RNA Extended Panel是通过RNAseq进行定量基因表达图谱分析的“从样本制备到数据解读”解决方案。这些panel结合了分子条形码技术和两步法PCR的文库制备方法，可无偏差、准确地定量数字RNA测序结果。
QIAseq Targeted RNA Extended Panels适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。
PCR duplicates are a major issue in targeted RNA sequencing for gene fusion detection, since – through PCR amplification – they turn unique RNA molecules into identical RNA molecules that cannot be distinguished from each other. This, in turn, results in the inability to confidently detect gene fusions. To overcome the issue of PCR duplicates, the QIAseq targeted RNAscan panels use digital sequencing by incorporating molecular barcodes into the starting RNA material before any amplification takes place – thereby preserving the uniqueness of the starting RNA molecules and overcoming the issues of not only PCR duplicates and amplification bias.
The entire workflow of the QIAseq Targeted RNAscan Panels – from extracted RNA to sequencing-ready libraries – can be completed in 9 hours. Extracted RNA is converted to cDNA, targets are molecularly barcoded and enriched, and libraries are constructed. Sequencing files can be fed into the QIAseq pipeline – a cloud-based data analysis pipeline – which will filter, map and align reads, as well as count unique molecular barcodes associated with targeted regions and call fusions. This data can then be fed into QCI for interpretation.
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