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EZ1 DNA Investigator Card

预设程序卡,用于配合EZ1 DNA Investigator Kit在BioRobot EZ1工作站上使用

  • 程序设置简单,插卡即用
  • 标准化操作流程,确保高重复性结果
  • 优化的实验方案,确保可靠纯化
  • 自动纯化基因组DNA

EZ1 DNA Investigator Card预装有纯化程序,配合EZ1 DNA Investigator Kit在BioRobot EZ1工作站上从法医检材和人身份鉴定样本中纯化DNA。

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Cat No./ID: 9016387
EZ1 DNA Investigator Card
Preprogrammed card for BioRobot EZ1 DNA Investigator protocols
EZ1 Cards应在BioRobot EZ1仪器上配合EZ1 Kits使用。请参见相关的EZ1 Kit使用说明和BioRobot EZ1用户手册,了解更多信息。

Product Details

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EZ1 DNA Investigator trace and “tip dance” protocols.
 
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Easy and efficient processing of solid materials with the “tip dance” protocol.
Papers from cigarette butts or 3 paper discs per sample were spotted with 10 ng DNA per sample. After proteinase K digestion, half of the samples were processed using the EZ1 DNA Investigator Kit with the "tip dance" protocol, without removing solid materials from the sample tubes (Tip dance). Solid materials were removed from the remaining half of the samples, which were then processed using the EZ1 DNA Investigator Kit with the standard trace protocol (Trace). DNA yields were quantified by real-time, quantitative PCR.
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Higher yields of concentrated DNA with the large-volume protocol.
 
Performance
Tip dance方式处理固体样本,操作简单

除了标准的微量方案,还可选用全自动tip dance实验方案,移液过程中滤嘴相对工作平台可来回移动。这样可在同一管中直接处理固体样本,如:拭子、组织、血斑或烟头。无需事先离心去除会阻塞滤嘴的固态物质(参见EZ1 DNA Investigator trace and “tip dance” protocols)。tip dance实验方案简化了操作步骤,在某些情况下能更有效的提取样本提高DNA的产量(参见Easy and efficient processing of solid materials with the "tip dance" protocol)。

经优化的实验方案,更大的样本容量

大体积实验方案可全自动处理的起始样本量多达500 µl。这不仅可以从含有低浓度DNA的稀释样本,如扩散的污渍中高效纯化DNA,而且能够纯化需要用大体积进行彻底裂解的样本。可处理大容量样本(与标准的微量方案有相同的洗脱体积),就可获得更高产量、更高浓度的DNA,在下游应用中有更高的灵敏度(参见Higher yields of concentrated DNA with the large-volume protocol)。

Principle
EZ1 Cards中存储有多种纯化程序,可直接使用,无需手动设置或输入数据。您可随时购买不同的EZ1 Cards,拓展BioRobot EZ1的应用范围。
Procedure
只需将存储您所需的实验程序的EZ1 Card插入仪器卡槽,即可开始反应。
Applications

在BioRobot EZ1上配合EZ1 DNA Investigator Kit和Card获得的高品质DNA适合多种法医检材、环境、生物防御、生物安全和其他应用测试应用,包括:

  • 基因分型,包括指纹分析和亲子鉴定分析
  • 从痕量样本中纯化DNA,如个案样本和犯罪现场样本
  • 从用于环境检测的滤器中提取DNA
  • 参考样本的常规分析

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试剂盒操作手册 (1)
For automated purification of DNA from forensic and biosecurity samples using EZ1 instruments
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