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mericon DNA Bacteria (Plus) Kit

从微生物富集培养物中提取高品质DNA
  • 快速、高效的裂解革兰氏阴性菌和阳性菌
  • 实验方案基于机械裂解与热裂解技术
  • 高效去除PCR抑制剂
mericon DNA Bacteria Kit和mericon DNA Bacteria Plus Kit能够从革兰氏阳性菌和阴性菌中提取高品质核酸。该试剂盒是QIAGEN丰富的食品检测产品线中的一部分,此外还有专门用于病原体和转基因生物DNA检测试剂盒和成分鉴定试剂盒。

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Cat No./ID: 69525
mericon DNA Bacteria Kit (100)
Fast Lysis Buffer
Cat No./ID: 69534
mericon DNA Bacteria Plus Kit (50)
50 Pathogen Lysis Tubes L, Fast Lysis Buffer
mericon DNA Bacteria Kit和mericon DNA Bacteria Plus Kits适用于食品、动物饲料和药品的分子生物学检测。该产品不适用于疾病的诊断、预防或治疗。

Product Details

9

Comparison of time to result of different methods for pathogen detection.

Delivering sensitive and highly specific results, real-time PCR analysis is also faster than conventional pathogen detection methods. Besides taking longer, immunoassays and traditional culture methods are less sensitive and more labor intensive.

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Highly sensitive pathogen detection.

Detection of pathogens was highly sensitive, even in difficult food matrices, such as peanut butter. Peanut butter was homogenized in buffered peptone water, spiked with <5 cfu of salmonella, and enriched for 20 h at 37°C. DNA was extracted from serially diluted samples of the enrichment culture using the mericon DNA Bacteria Kit and then tested with the mericon Salmonella Kit. Although the original inoculation was small, salmonella was still reliably detected at a dilution factor of 1:100,000.
9

Efficient DNA extraction from Gram-negative and Gram-positive bacteria.

[A] Samples of an enrichment culture of Listeria monocytogenes were diluted and subjected to the mechanical lysis protocol of the mericon DNA Bacteria Plus Kit and the thermal lysis protocol of the mericon DNA Bacteria Kit to evaluate lysis efficiency. For this Gram-positive bacterium, mechanical lysis delivered a more effective extraction, most evident at very low bacterial concentrations. [B] Diluted and undiluted samples of an enrichment culture of Salmonella enterica in milk and hazelnut spread were subjected to the mechanical and thermal lysis protocols. Overall, the efficiency of DNA extraction from Gram-negative bacteria was similar with both preparation protocols.
Performance
热裂解步骤采用mericon DNA Bacteria Kit,该试剂盒经过优化,能够轻松的裂解多数革兰氏阴性菌(例如Salmonella Camplylobacter spp.和Cronobacter spp.)。机械裂解使用mericon DNA Bacteria Plus Kit,为裂解Listeria spp.等革兰氏阳性菌提供理想环境(参见Efficient DNA extraction from Gram-negative or Gram-positive bacteria)。
Principle
当今检测食品病原体的主要方法为培养制备和/或ELISA方法检测。分子生物学方法,尤其是real-time PCR方法可为这些成熟的检测方法提供更多信息。此外,real-time PCR检测法能够更快获得结果(参见Comparison of time to result of different methods for pathogen detection),具有更高的灵敏度和特异性,可用于复杂食品样本(参见Highly sensitive pathogen detection)。real-time PCR是检测富有挑战性的病原体的安全方法。
Procedure

QIAGEN依据(采用微珠的)机械裂解和(通过加热的)热裂解技术,设计了专用实验方案,为不同类型细菌提供针对性裂解方案,使整个样本制备流程更高效。

热裂解适用于多数易裂解的革兰氏阴性菌,而机械样本制备则为难裂解的革兰氏阳性菌提供了理想的裂解条件。

mericon DNA Bacteria Kit和mericon DNA Bacteria Plus Kit提供专用试剂,能够极大提高裂解效率,稳定提取的DNA,去除可能影响下游应用的抑制剂。

Applications
mericon DNA Bacteria Kit和mericon DNA Bacteria Plus Kit适用于从病原体富集培养物中的革兰氏阴性菌(mericon DNA Bacteria Kit)和阳性菌(mericon DNA Bacteria Plus Kit)中提取总基因组DNA。

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