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PAXgene 96 Blood RNA Kit

高通量纯化全血样品胞内RNA

  • 同步纯化96个样品中的RNA
  • 标准化样品处理过程
  • 无需有机提取或沉淀
  • 纯化获得高品质胞内RNA

PAXgene 96 Blood RNA Kit可手工操作高通量纯化胞内RNA。配合PAXgene Blood RNA Tubes(由BD提供,货号762165),系统提供了一个样本分析前处理的标准方案,包括从收集、稳定到RNA纯化的全过程,适用于高通量的临床试验和其他大规模研究课题。

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Cat No./ID: 762331
PAXgene 96 Blood RNA Kit (4)
4 PAXgene 96 RNA Plates, 4 PAXgene 96 Filter Plates, Buffers, Proteinase K, RNase-free DNase Sets, AirPore Tape Sheets, Collection Vessels. To be used in conjunction with PAXgene Blood RNA Tubes.
Cat No./ID: 9238279
PAXgene 96 Incubator Block
Block for denaturation of eluates in PAXgene 96 procedures

仅限于科研应用,不适用于诊断,不为疾病的诊断、预防或治疗提供参考信息。


Product Details

9

Stabilization of gene expression.

Blood was collected and RNA was isolated using the PAXgene Blood RNA System (for RNA stabilization and isolation). The graph shows changes in expression of 12 genes after blood collection, measured using real-time RT-PCR. (Data kindly provided by Source Precision Medicine, Boulder, Colorado, USA.)

9
Detection of tyrosinase following blood storage.
Blood samples were collected from 6 different donors, spiked with tumor cells expressing tyrosinase, and stored for up to 10 days at room temperature. RT-PCR was used to measure levels of tyrosinase.  [A]  1 cell per 2.5 ml blood;  [B]  10 cells per 2.5 ml blood. WB: tyrosinase detection in RNA isolated from whole blood collected and stored in standard EDTA tubes. PAX: tyrosinase detection using the PAXgene Blood RNA System for sample collection, and RNA stabilization and isolation. (Data kindly provided by LMO, Munich, Germany.)
9
PAXgene 96 Blood RNA procedure.
RNA isolation begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed, and proteinase K is added to digest proteins. Alcohol is added to adjust binding conditions. Lysates are applied to the PAXgene 96 Filter Plate and centrifuged to remove cell debris. The lysate is then applied to a PAXgene 96 RNA Plate. Residual DNA is removed through a DNase I digestion. Remaining contaminants are removed in three efficient wash steps before pure RNA is eluted.
9

RNA isolation from blood using standard methods.

Blood was collected and RNA was isolated using standard methods (collection in EDTA tubes; no stabilization; RNA isolation using a guanidinium-based method). The graph shows changes in expression of 12 genes after blood collection, measured using real-time RT-PCR. (Data kindly provided by Source Precision Medicine, Boulder, Colorado, USA.)

Performance
PAXgene 96 Blood RNA Kit可高通量纯化人全血中的总RNA。配合PAXgene Blood RNA Tubes(BD提供,货号762165),提供从收集、稳定到纯化高品质RNA的完整分析前解决方案。灵敏的PAXgene 96 Blood RNA操作流程重复性好,2.5 ml人全血的一般产量为4–20 µg,将样本间的交叉污染风险降到最低。PAXgene Blood RNA Tubes提供的专有试剂可快速稳定RNA。这种试剂可阻止收集血液后在体外通常会发生的胞内RNA表达谱的剧烈变化(参见"RNA isolation from blood using standard methods"、"Stabilization of gene expression"和"Detection of tyrosinase following blood storage")。高通量步骤简单、快速、高效,平行处理96个样品可为您在临床实验研究中节约大量时间。
Principle

在PAXgene 96 Blood RNA System中,血液被直接收集到PAXgene Blood RNA Tubes中,使用PAXgene 96 Blood RNA Kit纯化RNA。PAXgene Blood RNA Tubes的专有试剂,可快速稳定RNA。血液样本在PAXgene Blood RNA Tubes中可室温保存5天。然后使用PAXgene 96 Blood RNA Kit提供的硅胶膜技术纯化RNA。得到的RNA可准确代表体内表达谱,适用于多种下游应用。

Procedure
血液样本(2.5 ml)收集在PAXgene Blood RNA Tubes(BD提供,货号762165)中,可在室温下保存或转移。在PAXgene 96 Blood RNA操作流程中,RNA的纯化开始于在PAXgene Blood RNA Tube中离心沉淀核酸。沉淀洗涤后加入蛋白酶K消化蛋白。加入乙醇调节结合条件。裂解液加入到PAXgene 96 Filter Plate中,离心去除细胞碎片。裂解液再加入到PAXgene 96 RNA Plate中(参见"PAXgene 96 Blood RNA procedure")。残留的DNA通过DNase I消化去除。剩余的污染物在3次有效的漂洗步骤中被去除,之后洗脱得到纯化的RNA。
Applications

从人全血中纯化的RNA可即用于一系列下游应用,包括:

  • 核酸扩增技术,如RT-PCR和NASBA
  • Expression-array和expression-chip分析
  • 定量RT-PCR,包括TaqMan技术
  • cDNA合成
  • RNase和S1核酸酶保护
  • Northern印记、点印记和狭缝印记分析
  • 引物延伸

Specifications

Features
Specifications
应用 PCR, real-time PCR, qPCR, NASBA, cDNA synthesis
符合CE/FDA/IVD Research use only
洗脱体积 45–60 µl
规格 96-well plate
主要样本类型 Whole blood
处理 Manual (centrifugation or vacuum)
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 Cellular RNA
样本量 2.5 ml
稳定 Yes
技术 Silica technology
每次运行或制备时间 <1.5 hours
产量 4–20 µg

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产品介绍与指南 (4)
试剂盒操作手册 (1)
For isolation of cellular RNA from whole blood Important: To be used only in conjunction with PAXgene Blood RNA tubes
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