text.skipToContent text.skipToNavigation

AllPrep DNA/RNA Mini Kit

从细胞和组织样本中同时纯化DNA和RNA

  • 从同一样本中获得高品质DNA和RNA
  • 从珍稀样本中得到较大量的DNA和RNA
  • 简短流畅的操作流程,实现快速纯化
  • 即用型DNA和RNA,用于多种下游应用
AllPrep DNA/RNA Mini Kit可以从同一个生物样本中同时纯化基因组DNA和总RNA。通过新型的AllPrep DNA离心柱纯化DNA,然后AllPrep柱流出液经过RNeasy MinElute离心柱纯化得到RNA。该试剂盒可在QIAcube全自动核酸纯化仪上自动运行,最多可处理107个细胞或30 mg的组织样本。

Buy Products

Cat No./ID: 80204
AllPrep DNA/RNA Mini Kit (50)

For 50 minipreps: AllPrep DNA Spin Columns, RNeasy Mini Spin Columns, Collection Tubes, RNase-Free Water and Buffers 

AllPrep DNA/RNA Mini Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Product Details

9
Wide dynamic range.
Genomic DNA and total RNA were purified from 10-fold serial dilutions of rat kidney homogenate using the AllPrep DNA/RNA Micro Kit; at each dilution, DNA was purified from duplicates, and RNA was purified from 4 replicates. [A] Real-time PCR analysis of the Pgk1 gene and [B] real-time RT-PCR analysis of the Jun transcript showed reliable quantification over a wide dynamic range, from 5 mg down to 50 ng tissue.
9
High-quality total RNA.
Total RNA was purified from 1 x 106 Jurkat cells using the AllPrep DNA/RNA Mini Kit and analyzed on the Agilent 2100 Bioanalyzer. The RIN value was 10, indicating intact RNA. M: marker; 18S: 18S rRNA; 28S: 28S rRNA.
9
Simultaneous purification of total RNA and genomic DNA.
Total RNA and genomic DNA were purified from 10 mg samples of rat liver using AllPrep technology (the AllPrep DNA/RNA Mini Kit). [A] Purified RNA samples were analyzed on a 1.2% formaldehyde agarose gel. [B] Purified DNA samples were analyzed on a 0.8% agarose gel. M: markers.
9
Multiplex PCR of 8 targets.
Genomic DNA was purified from mouse tissues using the AllPrep DNA/RNA Mini Kit. Multiplex PCR of 8 targets (192–1953 bp; GC content in the range of 19–61%) was performed using 100 ng DNA per sample. M: markers; NTC: no template control; Li: liver; K: kidney; Lu: lung.
9
AllPrep DNA/RNA procedure.
 
Performance
简单的流程可从同一样本中纯化高品质DNA和RNA。纯化得到的基因组DNA的平均长度为15–30 kb,适用于多重PCR(参见"Multiplex PCR of 8 targets")。纯化得到高品质的总RNA,RIN值接近10,表明RNA是完整的(参见"Simultaneous purification of total RNA and genomic DNA"和"High-quality total RNA")。AllPrep DNA/RNA纯化技术纯化得到的基因组DNA和总RNA在下游分析中可获得可靠的定量结果(参见"Wide dynamic range")。
Principle

AllPrep DNA/RNA Mini Kit用于从单个细胞或组织样本中纯化基因组DNA和总RNA。由于无需将样本分为两份单独进行纯化,可获得较大产量的DNA和RNA。经纯化的DNA和RNA可分别洗脱并直接用于多种下游应用。

Procedure

简单的流程可在35分钟内从同一样本中纯化高品质DNA和RNA(参见"AllPrep DNA/RNA procedure")。培养细胞和易裂解组织均可处理,应用新型AllPrep DNA离心柱纯化基因组DNA,RNeasy MinElute离心柱纯化总RNA。另可选择补充操作流程纯化蛋白质。纯化所得蛋白是变性的,可用于诸如SDS-PAGE、Western印迹和2D凝胶电泳等应用。对于经常从单一样本中纯化DNA、RNA和蛋白质的实验,可使用AllPrep DNA/RNA/Protein Mini Kit。

在Buffer RLT Plus(AllPrep DNA/RNA Mini Kit中提供)中破碎并匀浆组织时,可能出现很多泡沫。可在开始破碎和匀浆前,在Buffer RLT Plus加入Reagent DX(单独订购),终浓度为0.5%(v/v),即可有效消除泡沫。Reagent DX已经过精密测试,对于RNA纯度和诸如real-time RT-PCR等下游应用没有影响。添加了Reagent DX的Buffer RLT Plus可在室温(15–25ºC)下至少保存9个月。

Applications

纯化的基因组DNA的平均长度为15–30 kb,取决于匀浆情况,适合多种应用,包括:Southern印迹、斑点杂交和狭缝印迹、DNA甲基化分析、PCR和多重PCR。

经纯化的总RNA可即用于多种应用,包括:RT‑PCR和real‑time RT‑PCR、差异显示、cDNA合成、Northern印迹、斑点杂交和狭缝印迹、微阵列分析。

另可选择补充操作流程纯化蛋白质。纯化所得蛋白是变性的,可用于以下应用:SDS-PAGE 、Western印迹、2D凝胶电泳。

Specifications

Features
Specifications
应用 PCR, real-time PCR, microarray, blotting
洗脱体积 100 µl
规格 Spin column
主要样本类型 Cells, tissue
处理 Manual (centrifugation)
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 Genomic DNA, total RNA
样本量 1 x 10e7/30 mg
技术 Silica technology
每次运行或制备时间 35 minutes
产量 Varies

Product Resources

您无权限查看此资源

试剂盒操作手册 (1)
For simultaneous purification of genomic DNA and total RNA from the same animal cell or tissue sample
显示更多
参考文献
0
fragment fix placeholder

Product citations in scientific articles

See more details on Bioz