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AllPrep DNA/RNA 96 Kit

96孔模式同时纯化细胞或组织中的DNA和RNA

  • 60分钟内处理96个样本
  • 从同一样本中获得高品质的DNA和RNA
  • 96孔板模式,高效的操作流程
  • 高度标准化的操作流程
  • DNA和RNA的产量重复性高

AllPrep DNA/RNA 96 Kit适用于从96个样本中高通量纯化基因组DNA和总RNA。可以从同一个细胞或组织样本中同时纯化DNA和RNA。由于不需要将样本分成两份分别纯化,可以获得高产量的DNA和RNA。经纯化的DNA和RNA分别洗脱。

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Cat No./ID: 80311
AllPrep DNA/RNA 96 Kit (4)
For 4 x 96 preps: 4 AllPrep 96 DNA Plates, 4 RNeasy 96 Plates, Elution Microtubes CL, Caps, S-Blocks, AirPore Tape Sheets, RNase-Free Reagents and Buffers

AllPrep DNA/RNA 96 Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Product Details

9
AllPrep RNA 96-well plate.

The 96-well RNA purification plate of the AllPrep DNA/RNA 96 Kit.

9
Reproducible purification of DNA and RNA in 96-well format.
Genomic DNA and total RNA were purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. [A] Purified DNA samples were analyzed on a 0.8% agarose gel. [B] Purified RNA samples were analyzed on a 1.2% formaldehyde agarose gel. M: GelPilot Lambda HindIII Marker.
9
RNA with no genomic DNA contamination.
Total RNA was purified from rat kidney using the AllPrep DNA/RNA 96 Kit. Real-time RT-PCR analysis of the PGK1 transcript was performed using a primer-probe set that could amplify and detect both mRNA and genomic DNA sequences. The flat amplification plot for the no RT control (–RT) indicates the absence of genomic DNA.
9
High-quality DNA for reliable multiplex PCR analysis.
Genomic DNA was purified from mouse tissues using the AllPrep DNA/RNA 96 Kit. Six targets (195-1150 bp) were amplified by multiplex PCR and then analyzed on the QIAxcel system. Li: liver; Ki: kidney; Lu: lung; In: intestine; Sp: spleen; NTC: no template control (shows upper and lower markers).
9
AllPrep DNA/RNA procedure.
With the AllPrep DNA/RNA 96 Kit, an AllPrep 96 DNA plate and RNeasy 96 plate are used in the procedure. With the AllPrep DNA/RNA Micro Kit and AllPrep DNA/RNA Mini Kit, spin columns are used in the procedure. 
Performance

AllPrep DNA/RNA 96 Kit可从不同类型的组织中高效纯化高品质DNA和RNA(参见"Reproducible purification of DNA and RNA in 96-well format")。

使用AllPrep DNA/RNA 96 Kit从组织中纯化高品质RNA
大鼠组织 RIN值 *
9.4
9.5
9.6
胸腺 10.0
* 使用Agilent Bioanalyzer 2100进行分析,当RIN值接近10时,表示纯化得到了完整的RNA。

无需加入RNA酶或DNA酶进行消化(参见"RNA with no genomic DNA contamination"),经纯化的DNA适用于多种下游应用(参见"High-quality DNA for reliable multiplex PCR analysis")。

Principle

AllPrep DNA/RNA 96 Kit可纯化同一个样品的DNA和RNA,是遗传学和系统生物学样本制备的理想选择。可以从不同组织类型中高效纯化高品质的DNA和RNA并存档,无需加入RNA酶或DNA酶进行消化。可处理培养细胞和易裂解组织。该试剂盒是QIAGEN从一个样本中制备多种分析物的解决方案的一部分。

Procedure

简单的流程可从同一个样本中纯化高品质DNA和RNA(参见"AllPrep DNA/RNA procedure")。该试剂盒提供96孔纯化板(参见"AllPrep RNA 96-well plate";未显示DNA板),用于纯化DNA和RNA。通过离心(Centrifuge 4-16和Plate Rotor 2 x 96)或离心配合真空(QIAvac 96)操作,可方便、快速的处理样本。培养细胞和易裂解组织均可处理。

该试剂盒是QIAGEN从一个样本中制备多种分析物的解决方案的一部分。其中包括稳定组织中DNA、RNA和蛋白质的Allprotect Tissue Reagent以及稳定细胞中DNA和RNA的RNAprotect Cell Reagent。这两种试剂都可在室温下即刻稳定被分析物。对于组织,可应用TissueRuptor和TissueLyser进行低到高通量的快速破碎。

Applications

AllPrep DNA/RNA 96 Kit是遗传学和系统生物学样本制备的理想选择。纯化所得的基因组DNA的平均长度为15–30 kb,取决于匀浆情况,并且适合多种应用,包括:Southern印迹、斑点杂交和狭缝印迹、DNA甲基化分析、PCR和多重PCR。经纯化的总RNA可即用于多种应用,包括:RT‑PCR和real‑time RT‑PCR、差异显示、cDNA合成、Northern印迹、斑点杂交和狭缝印迹以及微阵列分析。

Specifications

Features
Specifications
应用 PCR, real-time PCR, microarray, blotting
洗脱体积 50 - 100 µl
规格 96-well plate
主要样本类型 Cultured cells, easy-to-lyse tissue
处理 Manual (centrifugation and/or vacuum)
纯化总RNA、miRNA、poly A+ mRNA、DNA或蛋白 Total RNA, DNA, miRNA (optional)
样本量 up to 2 x 10e6 cells / up to 10 mg tissue
技术 Silica technology
每次运行或制备时间 60 minutes
产量 varies

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