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QIAprep 96 Plus Kits

96孔板式纯化至多50 μg高品质质粒DNA

  • 持续获得至多50 μg的高产量
  • 纯化得到的产品适用于多种应用
  • 真空、离心和自动化多种处理方式可供选择
QIAprep 96 Plus Kits以96孔板式制备质粒DNA。使用QIAvac 96和/或离心机处理样本,或在BioRobot Universal System上自动处理。从5 ml E.coli 培养液中每孔可制备至多50 μg高品质质粒DNA(内毒素低于1 EU/μg DNA)。

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Cat No./ID: 27291
QIAprep 96 Plus Miniprep Kit (4)
For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, Flat-Bottom Blocks, S-Blocks, and Elution Microtubes; requires use of QIAvac 96 and Elution Microtube Adapter (available from QIAGEN Technical Service), or a centrifugation system suitable for 96-well blocks.
Cat No./ID: 962241
QIAprep 96 Plus BioRobot Kit (4)
For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, Flat-Bottom Blocks, S-Blocks, and Elution Microtubes; for use with the BioRobot Universal System

QIAprep 96 Plus Kits适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Product Details

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QIAprep 96 Plus Miniprep procedure.
 
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Superior plasmid DNA quality.
pCMVβ DNA was prepared using the QIAprep 96 Plus Miniprep Kit and kits from other suppliers. For agarose gel analysis, 150 ng (as determined by absorbance measurement) of each sample was used. With the QIAprep 96 Plus Miniprep Kit, a high percentage of supercoiled plasmid DNA (lower band) was observed and absorbance measurements and agarose gel analysis were comparable, indicating superior plasmid DNA quality. M: GelPilot 1 kb Plus Ladder (the most intense fragment corresponds to 100 ng).
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Superior performance compared to other suppliers.
E. coli DH5a strains carrying pCMVβ were grown in LB medium. Preps were performed according to the manufacturer's instructions. DNA was quantified using absorbance measurement and verified by agarose gel analysis. The QIAprep 96 Plus Miniprep Kit provided the highest yields compared to the other kits tested.
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High yields of plasmid DNA.
E. coli DH5a strains carrying pCMVβ were grown in 5 ml LB medium. Plasmid DNA was isolated manually using the QIAvac 96 and either the QIAprep 96 Plus Miniprep Kit or the QIAprep 96 Turbo Miniprep Kit or automated on the BioRobot Universal System using either the QIAprep 96 Plus BioRobot Kit or the QIAprep 96 Turbo BioRobot Kit. DNA was quantified using absorbance measurement and verified by agarose gel analysis. Higher yields were achieved with QIAprep 96 Plus Miniprep and BioRobot Kits.
Performance
高纯度和高产量

QIAprep 96 Plus Kits利用创新的方法制备高纯度DNA(参见"Superior plasmid DNA quality")。优化的操作流程确保能够持续获得多至50 μg的DNA。该试剂盒内含有独特的结合缓冲液,确保了特定的结合条件(参见"High yields of plasmid DNA" 和 "Superior performance compared to other suppliers")。

Principle
独特的试剂化学和Plasmid Plus 96板设计确保其高效的结合能力。中和后,通过TurboFilter 96板澄清裂解液。澄清后的裂解液中加入非离液结合缓冲液,使得质粒DNA充分结合到Plasmid Plus 96 板的膜上。独特的结合缓冲液确保特定的结合条件,得到的DNA品质相当于使用阴离子交换制备的DNA。 独特的试剂盒化学可从5 ml E. coli 培养液中每板纯化多至50 μg质粒DNA。
Procedure

QIAprep 96 Plus 利用96孔板式制备质粒。QIAprep 96 Plus Miniprep操作方法基于优化的碱裂解法。澄清裂解液后,只需简单的结合–洗涤–洗脱过程即可得到高浓度的质粒DNA,可直接用于下游应用。

方便、灵活处理

QIAprep 96 Plus Miniprep Kit使用QIAvac 96和/或离心机简便处理,也可在BioRobot Universal System上通过QIAprep 96 Plus BioRobot Kit全自动处理。优化的操作方法确保通过96孔板简便的进行质粒DNA制备。

Applications

QIAprep 96 Plus Kits纯化的质粒DNA高度适用于多种应用,包括:

  • 制备发夹载体(sh载体)
  • 酶修饰
  • 克隆
  • 限制性酶切
  • 测序 

Specifications

Features
Specifications
应用 Fluorescent and radioactive sequencing, cloning etc.
起始样本体积 1–10 ml culture volume
质粒类型 High-copy, low-copy, cosmid DNA
处理 Automated
每次处理样本量;通量 48–96 samples per run
技术 Silica technology
每次运行时间或处理样本数 <70 minutes
产量 <20 µg

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