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QIAGEN Plasmid Plus Kit

快速、便捷地纯化至多1 mg转染级质粒DNA

  • 20分钟内快速纯化至多24个样本
  • 利用真空装置,可同时处理多个样本
  • 低洗脱体积确保高浓度DNA
  • 独特的洗涤缓冲液确保较低的内毒素水平
  • 适合多种应用
QIAGEN Plasmid Plus Kit可超快速、大规模纯化获得至多10 mg的高纯度质粒DNA。使用真空装置可同时纯化至多24个样本,有效减少手动操作时间。低洗脱体积可获得高浓度质粒DNA,无需乙醇沉淀,即可直接应用。QIAGEN Plasmid Plus Kit含有新型的洗涤缓冲液,用于去除内毒素。纯化得到的质粒DNA适合多种应用,包括在敏感的细胞株中转染。

Buy Products

Cat No./ID: 12941
QIAGEN Plasmid Plus Midi Sample Kit (5)
QIAGEN Plasmid Plus Midi Sample Kit (5)
Cat No./ID: 12943
QIAGEN Plasmid Plus Midi Kit (25)
25 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Midi Cartridges
Cat No./ID: 12945
QIAGEN Plasmid Plus Midi Kit (100)
100 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges
Cat No./ID: 12963
QIAGEN Plasmid Plus Maxi Kit (25)
25 QIAGEN Plasmid Plus Maxi Columns, Extender Tubes, Reagents, Buffers, 25 QIAfilter Maxi Cartridges
Cat No./ID: 12965
QIAGEN Plasmid Plus Maxi Kit (100)
100 QIAGEN Plasmid Plus Maxi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Maxi Cartridges
Cat No./ID: 12981
QIAGEN Plasmid Plus Mega Kit (5)
5 QIAGEN Plasmid Plus Mega Columns, Extender Tubes, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges
Cat No./ID: 12991
QIAGEN Plasmid Plus Giga Kit (5)
5 QIAGEN Plasmid Plus Mega Columns, Extender Tubes, Reagents, Buffers, 5 QIAfilter Mega-Giga Cartridges
Cat No./ID: 19408
VacValves (24)
24 valves for use with the QIAvac 24 and QIAvac 24 Plus
Cat No./ID: 19418
QIAvac Holder
Holder for stabilizing QIAGEN Plasmid Plus Mega and Giga columns with Tube Extenders on the QIAvac 24 Plus

 


QIAGEN Plasmid Plus Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Product Details

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High yields are ensured with QIAGEN Plasmid Plus Kits (QPP).
Comparison of plasmid DNA yields using different methods — QIAGEN Plasmid Plus high-yield protocol (QPP), QIAGEN Plasmid Plus standard protocol (QPP), QIAGEN Plasmid Kit (QP), Supplier I, and Supplier S — is shown. Microgram DNA/ml biomass is indicated.
9
Analytical chromatogram.
Plasmid DNA (100 μg) purified using the QIAGEN Plasmid Plus Mega Kit was analyzed using HPLC on an analytical anion-exchange column to detect non-plasmid nucleic acid contamination (i.e., RNA or genomic DNA). No contamination is present in the plasmid prep.
9
QIAGEN Plasmid Plus procedure.
QIAGEN Plasmid Plus Kits provide a rapid and convenient method for large-scale plasmid preparation.
9
Endotoxin levels using different isolation methods.
QIAGEN Plasmid Plus Kits result in lower EU content levels than kits based on silica and anion-exchange technology from other suppliers. For EndoFree Plasmid Kits, the EU content is below detection range.
9
Endotoxin levels using QIAGEN Plasmid Plus Kits.
Purification per pellet-wet weight (g/l) for midi prep using Buffer ETR is shown. QIAGEN Plasmid Plus Technology generally results in low endotoxin levels. Buffer ETR technology further decreases these levels.
9
Efficient transfection in sensitive cell lines.
Plasmid pCMVβ DNA was prepared using the indicated preparation method with standard and high-yield (HY) protocol for QIAGEN Plasmid Plus Kits. Huh-7 cells were transfected using 200 ng plasmid DNA and 0.5 μl Attractene Transfection Reagent or 300 ng plasmid DNA and 0.75 μl Attractene Transfection Reagent.
Performance
稳定的质量和性能

QIAGEN Plasmid Plus技术可达到与阴离子交换技术相同的性能和品质。QIAGEN Plasmid Plus纯化柱独特的结合试剂确保得到高纯度质粒DNA。QIAGEN Plasmid Plus Maxi Kit提供高产量的实验方案和额外缓冲液,可获得多达10 mg高纯度质粒DNA,无RNA、蛋白质和基因组DNA等污染物(参见"High yields are ensured with QIAGEN Plasmid Plus Kits (QPP)"和"Analytical chromatogram")。

转染级质粒DNA,内毒素水平低

内毒素,又名脂多糖或LPS,在纯化质粒过程的裂解步骤释放,是革兰氏阴性菌如E. coli细胞膜的组成部分。内毒素包括非特异性激活免疫反应,是转染实验的非可控变量,影响实验结果和重复性,使其难以比较和解释。

该试剂提供的洗涤液将内毒素水平降至1 EU/μg DNA以下。(依宿主菌、培养基和细胞密度的不同而不同。如果细胞密度很高,内毒素水平也略高,在1–3 EU/μg DNA范围内。) 这使得到的质粒DNA适合多种应用,包括在敏感细胞系中转染(参见"Endotoxin levels using QIAGEN Plasmid Plus Kits"、"Endotoxin levels using different isolation methods"和"Efficient transfection in sensitive cell lines")。 

Principle

QIAGEN Plasmid Plus Kit进行大规模质粒纯化,方便快捷。独特设计的QIAGEN Plasmid Plus纯化柱和创新的结合试剂,使其只需简单的结合–洗涤–洗脱过程。QIAGEN Plasmid Plus Kit可在20分钟内实现快速大规模的质粒制备。无需乙醇沉淀可直接用于下游各种应用。该质粒DNA适用于要求小体积量的应用。

使用LyseBlue试剂,产量最大化

该试剂盒也包含LyseBlue指示剂,用于指示最佳的缓冲液混合状态。通过简单的颜色变化,就可避免常见的手动操作失误,防止细胞裂解不充分及SDS、基因组DNA和细胞碎片沉淀不完全。这种独特的缓冲液添加剂确保了细胞充分裂解和完全中和,使产量最大化。

Procedure
QIAGEN Plasmid Plus Kits提供的QIAfilter试剂条能快速澄清裂解液。一步即可将裂解液装入柱中,节约时间。使用真空装置(如QIAvac 24 Plus)抽吸澄清的裂解液及后续的洗涤缓冲液,使其流过QIAGEN Plasmid Plus离心柱。将膜洗涤和干燥后,即可通过离心获得高浓度DNA(参见"QIAGEN Plasmid Plus procedure")。
Applications

QIAGEN Plasmid Plus Kit纯化得到的转染级质粒DNA适合多种应用,例如:

  • 转染多数细胞系(敏感细胞系如Huh-7)
  • 限制性酶切
  • 克隆
  • 体外转录和翻译
  • 测序

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技术资讯 (2)
Technical Information
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Since QIAGEN launched the world’s first kit for purification of plasmid DNA, technologies have been developed to make plasmid DNA preparation even more convenient and reduce endotoxin levels in line with scientists’ needs in the laboratory. A lot of well-known companies have developed kits, and the choice of solutions available has never been greater. We compared popular advanced-technology kits to see their relative performance in speed, convenience of handling, yield and endotoxin levels.
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