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QIAGEN Plasmid Plus 96 Kits

用于在96孔板上纯化至多50 µg转染级质粒DNA
  • 纯化得到转染级质粒DNA,内毒素水平<1 EU/µg DNA
  • 产物DNA的纯度可与阴离子交换方法得到的纯度相同
  • 高产量(至多50 μg)
  • 操作便利、流程简单
  • 提供真空、离心和自动化处理操作方法
QIAGEN Plasmid Plus 96 Kits提供创新的方法通过96孔板纯化质粒。样本可在BioRobot Universal System上通过QIAvac 96和/或离心机处理或自动运行。使用专有的结合试剂,确保从5 ml E. coli培养液中每孔可纯化获得至多50 μg转染级质粒DNA。独特的内毒素去除缓冲液可使内毒素水平低至1 EU/μg DNA。

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Cat No./ID: 16181
QIAGEN Plasmid Plus 96 Miniprep Kit (4)
For 4 x 96 plasmid minipreps: TurboFilter 96 Plates, Plasmid Plus 96 Plates, Buffers, Reagents, Flat-Bottom Blocks, S-Blocks, and Elution Microtubes; requires use of QIAvac 96 and Elution Microtube Adapter (available from QIAGEN Technical Services), or a centrifugation system suitable for 96-well blocks
Cat No./ID: 960241
QIAGEN Plasmid Plus 96 BioRobot Kit (4)
For 4 x 96 plasmid minipreps: TurboFilter 96 Plates and Plasmid Plus 96 Plates, Buffers, Reagents, Flat-Bottom Blocks, S-Blocks, and Elution Microtubes; for use with the BioRobot Universal System

QIAGEN Plasmid Plus 96 Kits适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Product Details

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Highly efficient transfection into a sensitive cell line.
pCMVβ DNA was prepared using the indicated preparation method. Huh-7 cells (4 x 104) were transfected using 200 ng plasmid DNA and 0.75 μl Attractene Transfection Reagent. β-gal activity and protein content were measured after 48 hours. Plasmid DNA prepared with QIAGEN Plasmid Plus 96 Miniprep and BioRobot Kits resulted in highly efficient transfection into sensitive cell lines.
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High yields of plasmid DNA.
Using the QIAGEN Plasmid Plus 96 Miniprep Kit, pCMVβ plasmid DNA was purified from E. coli DH5a cultures grown in LB medium. DNA was quantified using absorbance measurement and verified by agarose gel analysis. High yields were achieved with the QIAGEN Plasmid Plus 96 Miniprep Kit.
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QIAGEN Plasmid Plus 96 procedure.
 
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Very low endotoxin levels.
pCMVβ plasmid DNA was purified from 1.3 ml and 5 ml E. coli DH10B cultures using the QIAGEN Plasmid Plus 96 Miniprep Kit, the QIAwell 96 Ultra Plasmid Kit, and kits from other suppliers. Endotoxin levels were determined using the Kinetic-QCL Kinetic Chromogenic Assay. For the EndoFree Plasmid Giga Kit, the endotoxin content was 0.0005 EU/μg DNA. EF: EndoFree Plasmid Giga Kit. QPP96: QIAGEN Plasmid Plus 96 Miniprep Kit. QW96: QIAwell 96 Ultra Plasmid Kit.
Performance
即用型质粒DNA,产量高且稳定

QIAGEN Plasmid Plus技术可达到与阴离子交换技术相同的性能和品质。可纯化获得多达50 μg的转染级质粒DNA(参见"High yields of plasmid DNA")。

极低的内毒素水平

该试剂盒应用内毒素去除缓冲液可有效降低内毒素水平至1 EU/μg DNA,得到的质粒DNA适合在敏感细胞系中转染(参见"Very low endotoxin levels"和"Highly efficient transfection into a sensitive cell line")。

Principle
QIAGEN Plasmid Plus 96 Kits基于QIAGEN Plasmid Plus技术,提供纯化质粒的创新方法。该操作流程基于非离液盐结合试剂,选择性好,且获得的DNA的品质与采用阴离子交换技术。裂解物澄清后,只需简单的结合-洗涤-洗脱过程,就可得到高浓度的质粒DNA,可直接用于下游应用。独特的试剂和Plasmid Plus 96板的设计确保了高效的结合能力。
Procedure
应用96孔板纯化质粒这种创新方法,只需简单的结合-洗涤-洗脱过程,就可得到高纯度转染级质粒DNA,(参见"QIAGEN Plasmid Plus 96 procedure")。

QIAGEN Plasmid Plus 96 Miniprep首先采用碱性裂解法,中和后,裂解物使用TurboFilter 96孔板澄清。澄清后的裂解物中加入非离液盐结合缓冲液确保质粒DNA充分与Plasmid Plus 96孔板的膜进行结合。独特的结合缓冲液可确保获得的DNA品质相当于采用阴离子交换技术制备的质粒DNA。内毒素去除缓冲液洗涤后,内毒素水平低至1 EU/μg DNA, 得到的DNA适合在敏感细胞系中转染。QIAGEN Plasmid Plus 96 Miniprep专有的结合试剂可从5 ml E. coli培养液中每孔可纯化获得多达50 μg转染级质粒DNA。

方便、灵活的操作选择

可在BioRobot Universal System上通过QIAGEN Plasmid Plus 96 BioRobot Kit进行自动纯化。也可将QIAvac 96与TurboFilter 96孔板配套使用,清洗裂解物,随后在Plasmid Plus 96板上进行DNA结合和洗涤。然后在QIAvac 96或兼容的离心机上进行烘干和洗脱。此外,整个流程都可在兼容的离心机上进行。

Applications

QIAGEN Plasmid Plus 96 Kits纯化得到的质粒DNA,适合多种应用,包括:

  • 转染,包括敏感细胞系转染
  • 制备短发夹载体(sh载体)
  • 酶修饰
  • 克隆
  • 限制性酶切

Specifications

Features
Specifications
应用 Fluorescent and radioactive sequencing, cloning etc.
起始样本体积 1–10 ml culture volume
质粒类型 High-copy, low-copy, cosmid DNA
处理 Automated
每次处理样本量;通量 48–96 samples per run
技术 Silica technology
每次运行时间或处理样本数 <70 minutes
产量 <20 µg

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