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HiSpeed Plasmid Maxi Kit

超快速纯化至多750 µg转染级纯质粒或柯斯质粒DNA

  1. 少于60分钟制备时间
  2. 无需离心即可澄清裂解液并进行异丙醇沉淀
  3. 在沉淀过程中不会损失DNA
  4. 可纯化至多750 µg高拷贝质粒DNA
  5. 使用LyseBlue,获得最佳的裂解效果和最大的DNA产量


HiSpeed Plasmid Kits使用阴离子交换技术,超快速、大规模纯化得到质粒DNA,无需离心。得到的DNA相当于两次CsCl梯度离心处理得到的纯度,适合转染级纯度的应用。

Buy Products

Cat No./ID: 1054575
HiSpeed Plasmid Giga EF Kit (5)
HiSpeed Plasmid Giga EF Kit (5)
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HiSpeed Plasmid Mega EF Kit (5)
HiSpeed Plasmid Mega EF Kit (5)
Cat No./ID: 19505
QIAvac HiSpeed LS
QIAvac HiSpeed LS

 


HiSpeed Plasmid Maxi Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


Product Details

9
QIAfilter Mega-Giga Cartridge

For fast purification of up to 10 mg transfection-grade plasmid or cosmid DNA

9
Bacterial cell wall.
 
9
HighSpeed Plasmid Giga EF procedure

Performance

HiSpeed Plasmid Kits提供QIAfilter Cartridges、HiSpeed Tips和QIAprecipitator模块,用于快速、大规模的制备质粒,无需真空底座。针筒规格的QIAfilter和QIAprecipitator模块取代了传统阴离子交换过程中的离心操作,质粒纯化更加方便、快捷。可从150–250 ml或50 µl – 150 ml的培养体积中纯化多至750 µg(Maxi)、或200 µg(Midi)的高拷贝质粒DNA(培养体积取决于质粒拷贝数、插入片段大小、宿主菌和培养基)。HiSpeed Tip支持高流速,确保更快地完成DNA结合、洗涤和洗脱。

此外,HiSpeed tips中新型的阴离子交换树脂广泛用于核酸的纯化。其独特的分离特性使得到的DNA的纯度相当于两次氯化铯梯度离心处理得到的纯度。


Principle

QIAfilter Cartridges(参见"QIAfilter Cartridge")是一种特制的过滤器,专用于取代细菌细胞碱裂解之后的离心步骤。QIAfilter Cartridges可去除SDS沉淀,只需很短的离心时间即可澄清细菌裂解液。预装的HiSpeed tips依靠重力流运行,确保不会流干,减少了质粒制备的手工操作时间。

独特的QIAprecipitator模块(参见"QIAprecipitator module")取代了异丙醇沉淀后传统的离心法纯化DNA。异丙醇混合液流过QIAprecipitator的时候DNA会结合上去,然后用TE缓冲液或水将DNA从QIAprecipitator洗脱下来。这一独特的模块降低了离心后转移上清液过程丢失DNA的风险。


Procedure
Neutralized bacterial lysates are incubated in the QIAfilter Cartridge and cleared in seconds by vacuum. At this stage, the Endotoxin Removal Buffer is added to the filtered lysate. No incubation is needed. The filtrate is applied to a HiSpeed tip for plasmid DNA purification (see figure “HighSpeed Plasmid Giga EF procedure”). Eluted DNA is mixed with isopropanol and applied to the QIAconcentrator using vacuum and washed. The concentrated and desalted DNA is then eluted from the QIAconcentrator directly into a fresh collection tube with TE buffer or water.
Applications

HiSpeed Plasmid Kits纯化得到超纯转染级纯DNA,适合于多种应用,包括:克隆、测序、转染和质粒介导的基因沉默等。

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